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Cellosaurus HEK293-EBNA (CVCL_6974)

[Text version]
Cell line name HEK293-EBNA
Synonyms 293 c18; 293c18; HEK 293 c18; HEK-293 c18; HEK293-EBNA1; HEK-293-EBNA; HEK 293-EBNA; HEK 293 EBNA; HEK293EBNA; 293 EBNA; 293-EBNA1; 293-EBNA; 293/EBNA; 293EBNA; EBNA-293; EBNA293; 293E; HEK293E; HEK/EBNA; HEK-EBNA; HEK.EBNA; 293/EBNA-1
Accession CVCL_6974
Secondary accession CVCL_6350
Resource Identification Initiative To cite this cell line use: HEK293-EBNA (RRID:CVCL_6974)
Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10852.
Characteristics: The use of recombinant protein expression vectors containing the EBV oriP in cell lines stably expressing EBV's EBNA1 protein significantly increases recombinant protein yield.
Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Discontinued: Thermofisher/Invitrogen; Catalog number R620-07.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Children:
CVCL_H365 (293 c18/DRD4)CVCL_H367 (293 c18/EP4)CVCL_A0DF (293C18P)
CVCL_D4BR (HEK293E_Clu AB1)CVCL_D4BS (HEK293E_Clu AB2)CVCL_D4BJ (HEK293E_Clu CC1)
CVCL_D4BK (HEK293E_Clu CC2)CVCL_D4BL (HEK293E_Clu CC3)CVCL_D4BM (HEK293E_Clu CC4)
CVCL_D4BN (HEK293E_Clu DD1)CVCL_D4BP (HEK293E_Clu DD2)CVCL_D4BQ (HEK293E_Clu DD3)
CVCL_D4BE (HEK293E_Clu TL1)CVCL_D4BF (HEK293E_Clu TL2)CVCL_D4BH (HEK293E_Clu TL2+3)
CVCL_D4BG (HEK293E_Clu TL3)CVCL_D4BI (HEK293E_Clu TL4)CVCL_D4BD (HEK293E_Clu WT)
CVCL_D4BT (HEK293E_Ecto short VLDLR-C-tag)CVCL_ZJ07 (LanthaScreen Akt HEK 293E)CVCL_ZJ06 (LanthaScreen PDCD4 HEK 293E)
CVCL_ZJ08 (LanthaScreen PRAS40 HEK 293E)CVCL_W713 (VEGF-D 293 clone 11)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC; CCRID

Markers:
AmelogeninX
CSF1PO11,12
D2S133819
D3S135815,17
D5S8188,9
D7S82011,12
D8S117912,14
D13S31712 (CCRID)
12,14 (ATCC)
D16S5399
D18S5118
D19S43318
D21S1128,30.2
FGA23
TH017,9.3
TPOX11
vWA16,19

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/2/cell-lines-detail-131.html
https://tools.thermofisher.com/content/sfs/manuals/293ebna_man.pdf
Publications

PubMed=21356792; DOI=10.1101/pdb.prot4976
Tom R., Bisson L., Durocher Y.
Culture of HEK293-EBNA1 cells for production of recombinant proteins.
CSH Protoc. 2008:Pdb.prot4976-Pdb.prot4976(2008)

DOI=10.13005/bpj/1414
Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-10852
CLS; 300264
Cell line databases/resources CLO; CLO_0001231
CCRID; 1101HUM-PUMC000152
FCS-free; 6-2-513-1-16-12
TOKU-E; 242
TOKU-E; 3609
TOKU-E; 3672
Anatomy/cell type resources BTO; BTO:0002974
Chemistry resources ChEMBL-Cells; CHEMBL3833702
PubChem_Cell_line; CVCL_6974
Encyclopedic resources Wikidata; Q54882445
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number26