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Cellosaurus HTR-8/SVneo (CVCL_7162)

[Text version]

Cell line name HTR-8/SVneo
Synonyms HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
Accession CVCL_7162
Resource Identification Initiative To cite this cell line use: HTR-8/SVneo (RRID:CVCL_7162)
Comments Part of: ENCODE project common cell types; tier 3.
Characteristics: Useful to study trophoblast and placental biology (ATCC).
Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV3-neo].
Omics: DNA methylation analysis.
Derived from sampling site: Placenta; trophoblast.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D728 (HTR-8)
Children:
CVCL_M689 (iC(3)-1)
Sex of cell Sex unspecified
Age at sampling 6-12FW
Category Transformed cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO12
D5S81812
D7S82012
D13S3179,12
D16S53913
TH016,9.3
TPOX8
vWA13,18

Run an STR similarity search on this cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/HTR8svn_Crawford_protocol.pdf
http://www.sonidel.com/sonidel/electroporation/nepa21-electroporator/cell-lines-htr-8-svneo-human-trophoblast-cells/
Publications

PubMed=7684692; DOI=10.1006/excr.1993.1139
Graham C.H., Hawley T.S., Hawley R.G., MacDougall J.R., Kerbel R.S., Khoo N., Lala P.K.
Establishment and characterization of first trimester human trophoblast cells with extended lifespan.
Exp. Cell Res. 206:204-211(1993)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

Cross-references
Cell line collections ATCC; CRL-3271
Cell line databases/resources TOKU-E; 4099
Ontologies BTO; BTO:0004125
EFO; EFO_0005709
Biological sample resources ENCODE; ENCBS331AAA
Gene expression databases GEO; GSM816644
Other Wikidata; Q54896677
Entry history
Entry creation04-Apr-2012
Last entry update12-Mar-2020
Version number19