ID   HTR-8/SVneo
AC   CVCL_7162
SY   HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
DR   BTO; BTO:0004125
DR   EFO; EFO_0005709
DR   ATCC; CRL-3271
DR   ENCODE; ENCBS331AAA
DR   GEO; GSM816644
DR   TOKU-E; 4099
DR   Ubigene; YC-C050
DR   Wikidata; Q54896677
RX   PubMed=7684692;
RX   PubMed=23325432;
RX   PubMed=28161053;
RX   PubMed=33096371;
RX   PubMed=33810901;
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/HTR8svn_Crawford_protocol.pdf
WW   http://www.sonidel.com/sonidel/electroporation/nepa21-electroporator/cell-lines-htr-8-svneo-human-trophoblast-cells/
CC   Part of: ENCODE project common cell types; tier 3.
CC   Characteristics: Useful to study trophoblast and placental biology (ATCC=CRL-3271).
CC   Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).
CC   Karyotypic information: Near triploid karyotype (PubMed=33096371).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=pSV3-neo].
CC   Omics: DNA methylation analysis.
CC   Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.
ST   Source(s): ATCC; PubMed=33810901
ST   Amelogenin: X
ST   CSF1PO: 12
ST   D10S1248: 16
ST   D12S391: 19
ST   D13S317: 9,12
ST   D16S539: 13
ST   D18S51: 13
ST   D19S433: 14,15
ST   D1S1656: 16,17.3
ST   D21S11: 29,30
ST   D22S1045: 15
ST   D2S1338: 23,24
ST   D2S441: 11
ST   D3S1358: 16
ST   D5S818: 12
ST   D7S820: 12
ST   D8S1179: 13,15
ST   FGA: 21,23
ST   Penta D: 9,12
ST   Penta E: 7,13
ST   SE33: 25.2,27.2
ST   TH01: 6,9.3
ST   TPOX: 8
ST   vWA: 13,18 (ATCC)
ST   vWA: 18 (PubMed=33810901)
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D728 ! HTR-8
SX   Sex unspecified
AG   6-12FW
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 25
//
RX   PubMed=7684692; DOI=10.1006/excr.1993.1139;
RA   Graham C.H., Hawley T.S., Hawley R.G., MacDougall J.R., Kerbel R.S.,
RA   Khoo N., Lala P.K.;
RT   "Establishment and characterization of first trimester human
RT   trophoblast cells with extended lifespan.";
RL   Exp. Cell Res. 206:204-211(1993).
//
RX   PubMed=23325432; DOI=10.1101/gr.147942.112;
RA   Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E.,
RA   Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A.,
RA   Crawford G.E., Absher D.M., Wold B.J., Myers R.M.;
RT   "Dynamic DNA methylation across diverse human cell lines and
RT   tissues.";
RL   Genome Res. 23:555-567(2013).
//
RX   PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007;
RA   Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.;
RT   "HTR-8/SVneo cell line contains a mixed population of cells.";
RL   Placenta 50:1-7(2017).
//
RX   PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011;
RA   Weber M., Weise A., Vasheghani F., Gohner C., Fitzgerald J.S.,
RA   Liehr T., Markert U.R.;
RT   "Cytogenomics of six human trophoblastic cell lines.";
RL   Placenta 103:72-75(2021).
//
RX   PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022;
RA   Pastuschek J., Nonn O., Gutierrez-Samudio R.N., Murrieta-Coxca J.M.,
RA   Muller J., Sanft J., Huppertz B., Markert U.R., Groten T.,
RA   Morales-Prieto D.M.;
RT   "Molecular characteristics of established trophoblast-derived cell
RT   lines.";
RL   Placenta 108:122-133(2021).
//