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Cellosaurus Hu609 (CVCL_8240)

[Text version]
Cell line name Hu609
Synonyms HU609; Hu 609; HU 609
Accession CVCL_8240
Resource Identification Initiative To cite this cell line use: Hu609 (RRID:CVCL_8240)
Comments Problematic cell line: Contaminated. Shown to be a J82 derivative (PubMed=7905254; PubMed=20143388). Originally thought to originate from the histologically normal ureter of a female patient with clear cell renal carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00131.
Population: Caucasian; Swedish.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Ser770Pro (c.2308T>C); ClinVar=VCV000140988; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro124Leu (c.371C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Asn212fs*1 (c.635_1212del578); Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 9884; RB1; Simple; c.2107-2A>G; ClinVar=VCV000013077; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu271Lys (c.811G>A); ClinVar=VCV000406566; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Lys320Asn (c.960G>C); Zygosity=Heterozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0359 (J82)
Children:
CVCL_8241 (Hu609T)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): PubMed=11416159

Markers:
AmelogeninX,Y
D8S11798,13
D18S5110,12
D21S1130,31
FGA20,24
TH019.3
vWA17,18

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=7262169; DOI=10.1016/0014-2964(81)90123-7
Vilien M., Wolf H., Rasmussen F.
Immunological characterization of cell lines establishing from malignant and normal human urothelium.
Eur. J. Cancer 17:321-327(1981)

PubMed=6546195; DOI=10.1007/BF00121494
Schroyens W., Mareel M.M., Dragonetti C.
In vitro invasiveness of human bladder cancer from cell lines and biopsy specimens.
Clin. Exp. Metastasis 1:153-162(1983)

PubMed=3708594
Masters J.R.W., Hepburn P.J., Walker L., Highman W.J., Trejdosiewicz L.K., Povey S., Parkar M., Hill B.T., Riddle P.N., Franks L.M.
Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.
Cancer Res. 46:3630-3636(1986)

PubMed=3800330
Debiec-Rychter M., Christensen B., Kieler J., Wang C.Y.
Chromosomal characterization and isoenzyme pattern of non-malignant and malignant human urothelial cell lines.
Anticancer Res. 6:1237-1244(1986)

PubMed=7905254
Christensen B., Hansen C., Kieler J., Schmidt J.
Identity of non-malignant human urothelial cell lines classified as transformation grade I (TGrI) and II (TGrII).
Anticancer Res. 13:2187-2191(1993)

PubMed=8105864; DOI=10.1038/bjc.1993.449
Christensen B., Hansen C., Debiec-Rychter M., Kieler J., Ottensen S., Schmidt J.
Identity of tumorigenic human urothelial cell lines and 'spontaneously' transformed sublines.
Br. J. Cancer 68:879-884(1993)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line databases/resources cancercelllines; CVCL_8240
Lonza; 245
Biological sample resources BioSample; SAMN03151802
Encyclopedic resources Wikidata; Q54896703
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number26