Cellosaurus NCOL-1 (CVCL_8375)

Cell line name NCOL-1
Synonyms Normal Colon-1
Accession CVCL_8375
Resource Identification Initiative To cite this cell line use: NCOL-1 (RRID:CVCL_8375)
Comments Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911, PubMed=20143388). Was originally thought to originate from a normal colonic mucosa sample.
Disease Colon adenocarcinoma (NCIt: C4349)
Derived from metastatic site: Left supraclavicular lymph node.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0399 (LoVo)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): PubMed=15771911

Markers:
AmelogeninX,Y
D16S5399,12
D18S5113,18
D19S43314,15 (PubMed=15771911_clone_a)
13,15 (PubMed=15771911_clone_b)
D21S1129,31.2 (PubMed=15771911_clone_a)
28,29,30.2,31.2 (PubMed=15771911_clone_b)
D2S133817,18
D3S135814,17
D8S117910 (PubMed=15771911_clone_a)
9,10 (PubMed=15771911_clone_b)
FGA17,20 (PubMed=15771911_clone_a)
18,20 (PubMed=15771911_clone_b)
SE3319.2,24.2 (PubMed=15771911_clone_a)
18.2,19.2,20.2,25.2 (PubMed=15771911_clone_b)
TH019.3
vWA17,18
Web pages http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf
Publications

PubMed=8812628; DOI=10.1006/jsre.1996.0323
Deveney C.W., Rand-Luby L., Rutten M.J., Luttropp C.A., Fowler W.M., Land J., Meichsner C.L., Farahmand M., Sheppard B.C., Crass R.A., Deveney K.E.
Establishment of human colonic epithelial cells in long-term culture.
J. Surg. Res. 64:161-169(1996)

PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023
Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M., Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W.
SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1.
Cancer Genet. Cytogenet. 158:84-87(2005)

PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003
Wenzel U., Daniel H.
Reconsidering cell line cross-contamination in NCOL-1.
Cancer Genet. Cytogenet. 163:95-96(2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Biological sample resources BioSample; SAMN03151825