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Cellosaurus 2fTGH-U3A (CVCL_9469)

[Text version]

Cell line name 2fTGH-U3A
Synonyms U3A
Accession CVCL_9469
Resource Identification Initiative To cite this cell line use: 2fTGH-U3A (RRID:CVCL_9469)
Comments Characteristics: Lacks expression of STAT1. Unresponsive to interferon-alpha and interferon-gamma (ECACC).
Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Transfected with: UniProtKB; P0A9M5; E.coli gpt.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Sequence variations Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0115 (2fTGH)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S8209,10
D13S31712,14
D16S5399,12
TH016
TPOX8
vWA14,19

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Publications

PubMed=1837150; DOI=10.1073/pnas.88.24.11455
McKendry R., John J., Flavell D., Muller M., Kerr I.M., Stark G.R.
High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.
Proc. Natl. Acad. Sci. U.S.A. 88:11455-11459(1991)

Cross-references
Cell line collections ECACC; 12021503
Ximbio; 151810
Other Wikidata; Q54584476
Entry history
Entry creation06-Jun-2012
Last entry update20-May-2021
Version number17