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Cellosaurus WA01 (CVCL_9771)

[Text version]

Cell line name WA01
Synonyms WA 01; WA-01; WA1; H1; H1-hESC; ESC H1; GE01; WAe001-A; WICELLe001-A
Accession CVCL_9771
Resource Identification Initiative To cite this cell line use: WA01 (RRID:CVCL_9771)
Comments Part of: ENCODE project common cell types; tier 1.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0043.
Registration: Swiss research registry; BAG-hES-IMP-0001.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-06.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: H3K27ac ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me2 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: H3K9me3 ChIP-seq epigenome analysis.
Omics: H4K20me1 ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
HLA typing Source: PubMed=16919167
Class I
HLA-AA*02:03
HLA-BB*08,35
HLA-CC*04,07
Class II
HLA-DQDQB1*02,05
HLA-DRDRB1*01,03
DRB3*01
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_UB70 (AI03e-DCXYFP)CVCL_UB71 (AI06e-SOX2YFP)CVCL_UB72 (AI07e-Timothy)
CVCL_UB73 (AI08e-PAX6YFP)CVCL_UB74 (AI09e-KCTD13a)CVCL_UB75 (AI10e-KCTD13b)
CVCL_UB76 (AI11e-OTX2YFP)CVCL_UB77 (AI12e-HOPX-CIT+/-)CVCL_UB78 (AI13e-HOPX-CIT+/+)
CVCL_WS14 (H1 iCas9)CVCL_C813 (H1.1)CVCL_DE08 (H1.NPC-i1)
CVCL_DE09 (H1.NPC-i2)CVCL_DE10 (H1.NPC-i3)CVCL_VJ25 (UCP1:mCherry H1 hES)
CVCL_VE15 (WAe001-A-10)CVCL_VE16 (WAe001-A-11)CVCL_LI07 (WAe001-A-12)
CVCL_RL77 (WAe001-A-13)CVCL_VE33 (WAe001-A-14)CVCL_VF26 (WAe001-A-15)
CVCL_RL89 (WAe001-A-16)CVCL_VN44 (WAe001-A-17)CVCL_RV18 (WAe001-A-18)
CVCL_VF82 (WAe001-A-19)CVCL_VE93 (WAe001-A-2)CVCL_UD91 (WAe001-A-20)
CVCL_UM34 (WAe001-A-21)CVCL_UL61 (WAe001-A-22)CVCL_UM35 (WAe001-A-23)
CVCL_UM36 (WAe001-A-24)CVCL_XD76 (WAe001-A-25)CVCL_XD77 (WAe001-A-26)
CVCL_XD78 (WAe001-A-27)CVCL_XD79 (WAe001-A-28)CVCL_QY38 (WAe001-A-3)
CVCL_XC42 (WAe001-A-31)CVCL_XC43 (WAe001-A-32)CVCL_QY75 (WAe001-A-4)
CVCL_QY76 (WAe001-A-5)CVCL_LK60 (WAe001-A-6)CVCL_QY77 (WAe001-A-7)
CVCL_QY78 (WAe001-A-8)CVCL_QY79 (WAe001-A-9)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=16919167; PubMed=24374290; PubMed=29034888; PubMed=29034901; WiCell

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818,19
D3S135815
D5S8189,11
D6S104312,14
D7S8208,12
D8S117912,13
D12S39120
D13S3178,11
D16S5399,13
D18S5117,18
D19S43313,15
D21S1128,32.2
FGA20,24
Penta D10,13
Penta E10,12
TH019.3
TPOX8,11
vWA15,17

Run an STR similarity search on this cell line
Web pages https://grants.nih.gov/stem_cells/registry/current.htm?id=29
https://stemcelldb.nih.gov/cellLineDetails.do?id=12
http://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M.
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Carpenter M.K., Rosler E.S., Fisk G.J., Brandenberger R., Ares X., Miura T., Lucero M., Rao M.S.
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R., Frane J.L., Crandall L.J., Daigh C.A., Conard K.R., Piekarczyk M.S., Llanas R.A., Thomson J.A.
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=16919167; DOI=10.1186/1741-7007-4-28
Josephson R., Sykes G., Liu Y., Ording C.J., Xu W., Zeng X., Shin S., Loring J.F., Maitra A., Rao M.S., Auerbach J.M.
A molecular scheme for improved characterization of human embryonic stem cell lines.
BMC Biol. 4:28-28(2006)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113
Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R., O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A., Morin R.D., Baross A., Zhao Y., Khattra J., Prabhu A.-L., Pandoh P., McDonald H., Asano J., Dhalla N., Ma K., Lee S., Ally A., Chahal N., Menzies S., Siddiqui A., Holt R., Jones S., Gerhard D.S., Thomson J.A., Eaves C.J., Marra M.A.
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M., Pedersen R., Pera M.F., Piekarczyk M.S., Pera R.A.R., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward D., Weaver T.A., Young L.A., Zhang W.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032
Bock C., Kiskinis E., Verstappen G., Gu H., Boulting G., Smith Z.D., Ziller M., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21714037; DOI=10.1002/stem.681
Ramirez J.-M., Gerbal-Chaloin S., Milhavet O., Qiang B., Becker F., Assou S., Lemaitre J.-M., Hamamah S., De Vos J.
Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
Stem Cells 29:1469-1474(2011)

PubMed=21983960; DOI=10.1038/nmeth.1699
Phanstiel D.H., Brumbaugh J., Wenger C.D., Tian S., Probasco M.D., Bailey D.J., Swaney D.L., Tervo M.A., Bolin J.M., Ruotti V., Stewart R., Thomson J.A., Coon J.J.
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=22514597; DOI=10.1371/journal.pone.0030743
Sandt C., Feraud O., Oudrhiri N., Bonnet M.-L., Meunier M.-C., Valogne Y., Bertrand A., Raphael M., Griscelli F., Turhan A.G., Dumas P., Bennaceur-Griscelli A.
Identification of spectral modifications occurring during reprogramming of somatic cells.
PLoS ONE 7:E30743-E30743(2012)

PubMed=22802639; DOI=10.1073/pnas.1209979109
Kajiwara M., Aoi T., Okita K., Takahashi R., Inoue H., Takayama N., Endo H., Eto K., Toguchida J., Uemoto S., Yamanaka S.
Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.
Proc. Natl. Acad. Sci. U.S.A. 109:12538-12543(2012)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002
Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J., Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=24259714; DOI=10.1073/pnas.1319061110
Koyanagi-Aoi M., Ohnuki M., Takahashi K., Okita K., Noma H., Sawamura Y., Teramoto I., Narita M., Sato Y., Ichisaka T., Amano N., Watanabe A., Morizane A., Yamada Y., Sato T., Takahashi J., Yamanaka S.
Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells.
Proc. Natl. Acad. Sci. U.S.A. 110:20569-20574(2013)

PubMed=24374290; DOI=10.1016/j.scr.2013.11.010
Mallon B.S., Hamilton R.S., Kozhich O.A., Johnson K.R., Fann Y.C., Rao M.S., Robey P.G.
Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
Stem Cell Res. 12:376-386(2014)

PubMed=27476965; DOI=10.1016/j.stem.2016.06.019
Nishizawa M., Chonabayashi K., Nomura M., Tanaka A., Nakamura M., Inagaki A., Nishikawa M., Takei I., Oishi A., Tanabe K., Ohnuki M., Yokota H., Koyanagi-Aoi M., Okita K., Watanabe A., Takaori-Kondo A., Yamanaka S., Yoshida Y.
Epigenetic variation between human induced pluripotent stem cell lines is an indicator of differentiation capacity.
Cell Stem Cell 19:341-354(2016)

PubMed=28445466; DOI=10.1038/nature22312
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.
Nature 545:229-233(2017)

PubMed=29034901; DOI=10.1016/j.scr.2017.08.016
Yuan F., Guo D.-S., Gao G., Liu Y.-L., Xu Y.-Y., Wu Y.-H., Yang F., Ke X.-R., Lai K.-Y., Hong L.-Q., Li Y.-X.
Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9.
Stem Cell Res. 24:89-93(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S., Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections WiCell; wa01
WiCell; wa01-cgmp-material
WiCell; wa01-matched-research-bank
Cell line databases/resources hPSCreg; WAe001-A
IGRhCellID; H1%20p61
ISCR; 87
SKIP; SKIP001933
Ontologies BTO; BTO:0006060
EFO; EFO_0003042
Biological sample resources 4DN; 4DNSRHNAF6M6
4DN; 4DNSRV3SKQ8M
BioSample; SAMN03471895
BioSamples; SAMEA7768168
ENCODE; ENCBS001VRA
ENCODE; ENCBS011NFB
ENCODE; ENCBS019EUF
ENCODE; ENCBS040WGM
ENCODE; ENCBS051SJH
ENCODE; ENCBS055BDT
ENCODE; ENCBS055DBZ
ENCODE; ENCBS060CKC
ENCODE; ENCBS081AUS
ENCODE; ENCBS083WOY
ENCODE; ENCBS086PQW
ENCODE; ENCBS103ZIA
ENCODE; ENCBS111ENC
ENCODE; ENCBS131GSD
ENCODE; ENCBS153GON
ENCODE; ENCBS215WGD
ENCODE; ENCBS244HDB
ENCODE; ENCBS262EHC
ENCODE; ENCBS270AGK
ENCODE; ENCBS282MUP
ENCODE; ENCBS285QSX
ENCODE; ENCBS299BNQ
ENCODE; ENCBS299NOL
ENCODE; ENCBS309DZE
ENCODE; ENCBS345JIP
ENCODE; ENCBS368BPP
ENCODE; ENCBS380OBH
ENCODE; ENCBS409PYK
ENCODE; ENCBS440MTS
ENCODE; ENCBS479XUT
ENCODE; ENCBS428NIG
ENCODE; ENCBS484NGW
ENCODE; ENCBS497CZZ
ENCODE; ENCBS513BIK
ENCODE; ENCBS534NMZ
ENCODE; ENCBS559QNR
ENCODE; ENCBS568FYY
ENCODE; ENCBS570AYT
ENCODE; ENCBS582QGL
ENCODE; ENCBS614LXI
ENCODE; ENCBS626BQK
ENCODE; ENCBS649THQ
ENCODE; ENCBS661AAA
ENCODE; ENCBS665AAA
ENCODE; ENCBS668AAA
ENCODE; ENCBS670AAA
ENCODE; ENCBS704NOS
ENCODE; ENCBS706WYC
ENCODE; ENCBS708BLT
ENCODE; ENCBS711AAA
ENCODE; ENCBS716AAA
ENCODE; ENCBS716FMR
ENCODE; ENCBS717WTB
ENCODE; ENCBS718AAA
ENCODE; ENCBS720AAA
ENCODE; ENCBS731AAA
ENCODE; ENCBS732AAA
ENCODE; ENCBS733AAA
ENCODE; ENCBS734AAA
ENCODE; ENCBS739PSV
ENCODE; ENCBS740OND
ENCODE; ENCBS780AAA
ENCODE; ENCBS780WAB
ENCODE; ENCBS797AAA
ENCODE; ENCBS802AAA
ENCODE; ENCBS803AAA
ENCODE; ENCBS819TBN
ENCODE; ENCBS820AAA
ENCODE; ENCBS847AAA
ENCODE; ENCBS854AAA
ENCODE; ENCBS855AAA
ENCODE; ENCBS874KZJ
ENCODE; ENCBS887ZGJ
ENCODE; ENCBS899KQD
ENCODE; ENCBS899NYB
ENCODE; ENCBS929BKG
ENCODE; ENCBS945MCY
ENCODE; ENCBS977TKX
ENCODE; ENCBS982HXP
ENCODE; ENCBS984IPH
Gene expression databases GEO; GSM347921
GEO; GSM409307
GEO; GSM409308
GEO; GSM409312
GEO; GSM410807
GEO; GSM410808
GEO; GSM428286
GEO; GSM428289
GEO; GSM428291
GEO; GSM428295
GEO; GSM428296
GEO; GSM429321
GEO; GSM429322
GEO; GSM429323
GEO; GSM432392
GEO; GSM432685
GEO; GSM432686
GEO; GSM433167
GEO; GSM433170
GEO; GSM433171
GEO; GSM433174
GEO; GSM433176
GEO; GSM433177
GEO; GSM433179
GEO; GSM434762
GEO; GSM434776
GEO; GSM434785
GEO; GSM438361
GEO; GSM438362
GEO; GSM450236
GEO; GSM450239
GEO; GSM450266
GEO; GSM450268
GEO; GSM450270
GEO; GSM466732
GEO; GSM466734
GEO; GSM466737
GEO; GSM466739
GEO; GSM469971
GEO; GSM484408
GEO; GSM537679
GEO; GSM537680
GEO; GSM537681
GEO; GSM537682
GEO; GSM537683
GEO; GSM537684
GEO; GSM537685
GEO; GSM543005
GEO; GSM579898
GEO; GSM579899
GEO; GSM602251
GEO; GSM602252
GEO; GSM602253
GEO; GSM637775
GEO; GSM733657
GEO; GSM733670
GEO; GSM733672
GEO; GSM733687
GEO; GSM733718
GEO; GSM733725
GEO; GSM733748
GEO; GSM733773
GEO; GSM733782
GEO; GSM736582
GEO; GSM803345
GEO; GSM803365
GEO; GSM803366
GEO; GSM803377
GEO; GSM803382
GEO; GSM803396
GEO; GSM803405
GEO; GSM803419
GEO; GSM803424
GEO; GSM803425
GEO; GSM803426
GEO; GSM803427
GEO; GSM803428
GEO; GSM803430
GEO; GSM803437
GEO; GSM803438
GEO; GSM803450
GEO; GSM803466
GEO; GSM803476
GEO; GSM803484
GEO; GSM803501
GEO; GSM803506
GEO; GSM803512
GEO; GSM803513
GEO; GSM803529
GEO; GSM803542
GEO; GSM816632
GEO; GSM822274
GEO; GSM822297
GEO; GSM822300
GEO; GSM856729
GEO; GSM935289
GEO; GSM935292
GEO; GSM935293
GEO; GSM935295
GEO; GSM935296
GEO; GSM935297
GEO; GSM935303
GEO; GSM935308
GEO; GSM935348
GEO; GSM935352
GEO; GSM935379
GEO; GSM935380
GEO; GSM935382
GEO; GSM935434
GEO; GSM935463
GEO; GSM935509
GEO; GSM935514
GEO; GSM935517
GEO; GSM935580
GEO; GSM935581
GEO; GSM935614
GEO; GSM1003444
GEO; GSM1003446
GEO; GSM1003451
GEO; GSM1003457
GEO; GSM1003472
GEO; GSM1003473
GEO; GSM1003479
GEO; GSM1003509
GEO; GSM1003513
GEO; GSM1003524
GEO; GSM1003547
GEO; GSM1003571
GEO; GSM1003572
GEO; GSM1003573
GEO; GSM1003579
GEO; GSM1003584
GEO; GSM1003585
GEO; GSM1003619
GEO; GSM1010742
GEO; GSM1010743
GEO; GSM1010776
GEO; GSM1010845
GEO; GSM1010896
GEO; GSM1010898
GEO; GSM1010899
GEO; GSM1040242
GEO; GSM1040312
GEO; GSM1153529
GEO; GSM1489248
GEO; GSM1489459
GEO; GSM1582545
GEO; GSM1658376
Other Wikidata; Q54993510
Proteomic databases PRIDE; PXD003903
Entry history
Entry creation06-Jun-2012
Last entry updated05-Jul-2019
Version number24