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Cellosaurus WA07 (CVCL_9772)

[Text version]

Cell line name WA07
Synonyms WA 07; WA-07; WA7; H7; H7 GE07; H7-hESC; ES-WA7; WAe002-A; WICELLe002-A; WAe007-A
Accession CVCL_9772
Resource Identification Initiative To cite this cell line use: WA07 (RRID:CVCL_9772)
Comments Part of: ENCODE project common cell types; tier 3.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0061.
Registration: Swiss research registry; BAG-hES-IMP-0034.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-18.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Miscellaneous: Sibling to WA13 (CVCL_9774) and WA14 (CVCL_9775).
HLA typing Source: PubMed=16919167
Class I
HLA-AA*02:25
HLA-BB*18,35
HLA-CC*04,12
Class II
HLA-DQDQB1*02,03
HLA-DRDRB1*07,11
DRB3*02
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S798 (H7.s14)CVCL_S799 (H7.s6)CVCL_S800 (H7.s9)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=16919167; WiCell

Markers:
AmelogeninX
CSF1PO12
D5S81811,13
D7S82010,11
D13S31711,12
D16S53912,13
TH016
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages https://grants.nih.gov/stem_cells/registry/current.htm?id=413
http://genome.ucsc.edu/ENCODE/protocols/cell/human/H7-hESC_StamAndCrawford_protocol.pdf
https://stemcelldb.nih.gov/cellLineDetails.do?id=3
http://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M.
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Carpenter M.K., Rosler E.S., Fisk G.J., Brandenberger R., Ares X., Miura T., Lucero M., Rao M.S.
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=15345125; DOI=10.1089/scd.2004.13.325
Draper J.S., Moore H.D., Ruban L.N., Gokhale P.J., Andrews P.W.
Culture and characterization of human embryonic stem cells.
Stem Cells Dev. 13:325-336(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R., Frane J.L., Crandall L.J., Daigh C.A., Conard K.R., Piekarczyk M.S., Llanas R.A., Thomson J.A.
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=16919167; DOI=10.1186/1741-7007-4-28
Josephson R., Sykes G., Liu Y., Ording C.J., Xu W., Zeng X., Shin S., Loring J.F., Maitra A., Rao M.S., Auerbach J.M.
A molecular scheme for improved characterization of human embryonic stem cell lines.
BMC Biol. 4:28-28(2006)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113
Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R., O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A., Morin R.D., Baross A., Zhao Y., Khattra J., Prabhu A.-L., Pandoh P., McDonald H., Asano J., Dhalla N., Ma K., Lee S., Ally A., Chahal N., Menzies S., Siddiqui A., Holt R., Jones S., Gerhard D.S., Thomson J.A., Eaves C.J., Marra M.A.
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M., Pedersen R., Pera M.F., Piekarczyk M.S., Pera R.A.R., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward D., Weaver T.A., Young L.A., Zhang W.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032
Bock C., Kiskinis E., Verstappen G., Gu H., Boulting G., Smith Z.D., Ziller M., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21983960; DOI=10.1038/nmeth.1699
Phanstiel D.H., Brumbaugh J., Wenger C.D., Tian S., Probasco M.D., Bailey D.J., Swaney D.L., Tervo M.A., Bolin J.M., Ruotti V., Stewart R., Thomson J.A., Coon J.J.
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=22315618; DOI=10.1155/2012/875059
Pekkanen-Mattila M., Ojala M., Kerkela E., Rajala K., Skottman H., Aalto-Setala K.
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Stem Cells Int. 2012:875059-875059(2012)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002
Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J., Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=23341440; DOI=10.5966/sctm.2012-0047
Toivonen S., Ojala M., Hyysalo A., Ilmarinen T., Rajala K., Pekkanen-Mattila M., Aanismaa R., Lundin K., Palgi J., Weltner J., Trokovic R., Silvennoinen O., Skottman H., Narkilahti S., Aalto-Setala K., Otonkoski T.
Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines.
Stem Cells Transl. Med. 2:83-93(2013)

PubMed=28445466; DOI=10.1038/nature22312
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S., Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections WiCell; wa07
Cell line databases/resources hPSCreg; WAe007-A
IGRhCellID; H7%20p91
ISCR; 88
SKIP; SKIP001934
Ontologies BTO; BTO:0006061
EFO; EFO_0005904
Biological sample resources BioSample; SAMN03471896
ENCODE; ENCBS017AOD
ENCODE; ENCBS052WSJ
ENCODE; ENCBS058YII
ENCODE; ENCBS068KXI
ENCODE; ENCBS291AAA
ENCODE; ENCBS292AAA
ENCODE; ENCBS293AAA
ENCODE; ENCBS297CQV
ENCODE; ENCBS311AAZ
ENCODE; ENCBS465FZV
ENCODE; ENCBS559YVY
ENCODE; ENCBS611ORI
ENCODE; ENCBS624XJG
ENCODE; ENCBS721YNV
ENCODE; ENCBS851RDN
ENCODE; ENCBS884CKU
ENCODE; ENCBS920PVU
ENCODE; ENCBS962QAF
Gene expression databases GEO; GSM347922
GEO; GSM500935
GEO; GSM510581
GEO; GSM537623
GEO; GSM537639
GEO; GSM537640
GEO; GSM537641
GEO; GSM537657
GEO; GSM580005
GEO; GSM621347
GEO; GSM621353
GEO; GSM637776
GEO; GSM669896
GEO; GSM669926
GEO; GSM736610
GEO; GSM736638
GEO; GSM856730
GEO; GSM945183
GEO; GSM945184
GEO; GSM945185
GEO; GSM945186
GEO; GSM981283
GEO; GSM1008596
GEO; GSM1215275
GEO; GSM1215276
Other Wikidata; Q54993513
Entry history
Entry creation06-Jun-2012
Last entry updated05-Jul-2019
Version number24