ID   WA09
AC   CVCL_9773
SY   WA 09; WA-09; WA9; H9; H9.hESCs; H9 ESC; H9 hES; H9ES; GE09; WAe003-A; WICELLe003-A; WAe009-A
DR   BTO; BTO:0006062
DR   4DN; 4DNSR9WBB58Z
DR   BioSample; SAMN03471903
DR   BioSamples; SAMEA7769668
DR   ENCODE; ENCBS013BRF
DR   ENCODE; ENCBS061KRP
DR   ENCODE; ENCBS140HIH
DR   ENCODE; ENCBS277KDR
DR   ENCODE; ENCBS298AAA
DR   ENCODE; ENCBS299AAA
DR   ENCODE; ENCBS300AAA
DR   ENCODE; ENCBS315DEY
DR   ENCODE; ENCBS375GFI
DR   ENCODE; ENCBS377MXB
DR   ENCODE; ENCBS399JWQ
DR   ENCODE; ENCBS417MAQ
DR   ENCODE; ENCBS477SFO
DR   ENCODE; ENCBS532LWE
DR   ENCODE; ENCBS616QKE
DR   ENCODE; ENCBS656LSW
DR   ENCODE; ENCBS692FVF
DR   ENCODE; ENCBS703ZDS
DR   ENCODE; ENCBS741LLD
DR   ENCODE; ENCBS769EPD
DR   ENCODE; ENCBS808UWK
DR   ENCODE; ENCBS853WXF
DR   GEO; GSM347923
DR   GEO; GSM500936
DR   GEO; GSM579901
DR   GEO; GSM579902
DR   GEO; GSM603054
DR   GEO; GSM603055
DR   GEO; GSM605307
DR   GEO; GSM605310
DR   GEO; GSM605314
DR   GEO; GSM605316
DR   GEO; GSM605324
DR   GEO; GSM616127
DR   GEO; GSM616128
DR   GEO; GSM616129
DR   GEO; GSM627785
DR   GEO; GSM637777
DR   GEO; GSM665037
DR   GEO; GSM667608
DR   GEO; GSM667609
DR   GEO; GSM667610
DR   GEO; GSM667611
DR   GEO; GSM667612
DR   GEO; GSM667613
DR   GEO; GSM667616
DR   GEO; GSM667619
DR   GEO; GSM667620
DR   GEO; GSM667621
DR   GEO; GSM667622
DR   GEO; GSM667623
DR   GEO; GSM667625
DR   GEO; GSM667626
DR   GEO; GSM667628
DR   GEO; GSM667629
DR   GEO; GSM667630
DR   GEO; GSM667631
DR   GEO; GSM667632
DR   GEO; GSM667633
DR   GEO; GSM667634
DR   GEO; GSM667635
DR   GEO; GSM667636
DR   GEO; GSM667637
DR   GEO; GSM667638
DR   GEO; GSM667639
DR   GEO; GSM667640
DR   GEO; GSM667643
DR   GEO; GSM669899
DR   GEO; GSM669901
DR   GEO; GSM670053
DR   GEO; GSM674603
DR   GEO; GSM706044
DR   GEO; GSM706059
DR   GEO; GSM706060
DR   GEO; GSM706061
DR   GEO; GSM706062
DR   GEO; GSM706063
DR   GEO; GSM706064
DR   GEO; GSM706066
DR   GEO; GSM706071
DR   GEO; GSM706076
DR   GEO; GSM816629
DR   GEO; GSM856731
DR   GEO; GSM927236
DR   GEO; GSM997555
DR   GEO; GSM997556
DR   GEO; GSM997557
DR   GEO; GSM1040243
DR   GEO; GSM1040313
DR   GEO; GSM1215278
DR   GEO; GSM1215279
DR   GEO; GSM1227088
DR   GEO; GSM1489249
DR   GEO; GSM1489460
DR   GEO; GSM1658377
DR   hPSCreg; WAe009-A
DR   IGRhCellID; H9%20p25-2
DR   IGRhCellID; H9%20p34
DR   ISCR; 89
DR   LINCS_LDP; ES-1001
DR   PRIDE; PXD003903
DR   PRIDE; PXD006271
DR   SKIP; SKIP001452
DR   SKIP; SKIP001935
DR   TOKU-E; 4223
DR   WiCell; wa09
DR   WiCell; wa09-cgmp-material
DR   WiCell; wa09-matched-research
DR   Wikidata; Q27555433
RX   PubMed=9804556;
RX   PubMed=14745950;
RX   PubMed=16388305;
RX   PubMed=16919167;
RX   PubMed=17570852;
RX   PubMed=17572666;
RX   PubMed=21295703;
RX   PubMed=21983960;
RX   PubMed=22514597;
RX   PubMed=22802639;
RX   PubMed=23117585;
RX   PubMed=24259714;
RX   PubMed=27476965;
RX   PubMed=28435121;
RX   PubMed=28445466;
RX   PubMed=29246576;
RX   PubMed=30388422;
WW   https://grants.nih.gov/stem_cells/registry/current.htm?id=414
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/BG02ES_and_H9ES_Myers_protocols.pdf
WW   https://strap.nci.nih.gov/celline_detail.php?sample_id=88
WW   https://stemcelldb.nih.gov/cellLineDetails.do?id=13
WW   http://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
CC   Group: Clinical grade hESC cell line.
CC   Part of: ENCODE project common cell types; tier 3.
CC   From: University of Wisconsin; Madison; USA.
CC   Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0062.
CC   Registration: Swiss research registry; BAG-hES-IMP-0016.
CC   Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-06.
CC   HLA typing: A*02:03; B*35,44; C*04,07; DQB1*05,06; DRB1*15,16:01; DRB5*01 (PubMed=16919167).
CC   Sequence variation: TP53 p.Pro151Ser (c.451C>T), p.Arg181His (c.542G>A), p.Arg248Gln (c.743G>A) and p.Arg267Trp (c.799C>T); somatic mutations acquired during proliferation (PubMed=28445466).
CC   Omics: Deep exome analysis.
CC   Omics: Deep phosphoproteome analysis.
CC   Omics: Deep proteome analysis.
CC   Omics: DNA methylation analysis.
CC   Omics: Genome sequenced.
CC   Omics: H2AK5ac ChIP-seq epigenome analysis.
CC   Omics: H2BK12ac ChIP-seq epigenome analysis.
CC   Omics: H2BK15ac ChIP-seq epigenome analysis.
CC   Omics: H2BK5ac ChIP-seq epigenome analysis.
CC   Omics: H3K18ac ChIP-seq epigenome analysis.
CC   Omics: H3K23ac ChIP-seq epigenome analysis.
CC   Omics: H3K23me2 ChIP-seq epigenome analysis.
CC   Omics: H3K27ac ChIP-seq epigenome analysis.
CC   Omics: H3K27me3 ChIP-seq epigenome analysis.
CC   Omics: H3K36me3 ChIP-seq epigenome analysis.
CC   Omics: H3K4ac ChIP-seq epigenome analysis.
CC   Omics: H3K4me1 ChIP-seq epigenome analysis.
CC   Omics: H3K4me2 ChIP-seq epigenome analysis.
CC   Omics: H3K4me3 ChIP-seq epigenome analysis.
CC   Omics: H3K56ac ChIP-seq epigenome analysis.
CC   Omics: H3K79me1 ChIP-seq epigenome analysis.
CC   Omics: H3K79me2 ChIP-seq epigenome analysis.
CC   Omics: H3K9ac ChIP-seq epigenome analysis.
CC   Omics: H3K9me3 ChIP-seq epigenome analysis.
CC   Omics: H4K20me1 ChIP-seq epigenome analysis.
CC   Omics: H4K5ac ChIP-seq epigenome analysis.
CC   Omics: H4K8ac ChIP-seq epigenome analysis.
CC   Omics: H4K91ac ChIP-seq epigenome analysis.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis.
CC   Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
ST   Source(s): PubMed=16919167; PubMed=29246576; WiCell
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 9
ST   D16S539: 12,13
ST   D18S51: 13
ST   D19S433: 12,15
ST   D21S11: 30
ST   D2S1338: 18,24
ST   D3S1358: 13,16
ST   D5S818: 11,12
ST   D7S820: 9,11
ST   D8S1179: 8,14
ST   FGA: 26,28
ST   Penta D: 9,13
ST   Penta E: 11,14
ST   TH01: 9.3
ST   TPOX: 10,11
ST   vWA: 17
OX   NCBI_TaxID=9606; ! Homo sapiens
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 06-06-12; Last updated: 05-07-19; Version: 24
//
RX   PubMed=9804556; DOI=10.1126/science.282.5391.1145;
RA   Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A.,
RA   Swiergiel J.J., Marshall V.S., Jones J.M.;
RT   "Embryonic stem cell lines derived from human blastocysts.";
RL   Science 282:1145-1147(1998).
//
RX   PubMed=14745950; DOI=10.1002/dvdy.10431;
RA   Carpenter M.K., Rosler E.S., Fisk G.J., Brandenberger R., Ares X.,
RA   Miura T., Lucero M., Rao M.S.;
RT   "Properties of four human embryonic stem cell lines maintained in a
RT   feeder-free culture system.";
RL   Dev. Dyn. 229:243-258(2004).
//
RX   PubMed=16388305; DOI=10.1038/nbt1177;
RA   Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R.,
RA   Frane J.L., Crandall L.J., Daigh C.A., Conard K.R., Piekarczyk M.S.,
RA   Llanas R.A., Thomson J.A.;
RT   "Derivation of human embryonic stem cells in defined conditions.";
RL   Nat. Biotechnol. 24:185-187(2006).
//
RX   PubMed=16919167; DOI=10.1186/1741-7007-4-28;
RA   Josephson R., Sykes G., Liu Y., Ording C.J., Xu W., Zeng X., Shin S.,
RA   Loring J.F., Maitra A., Rao M.S., Auerbach J.M.;
RT   "A molecular scheme for improved characterization of human embryonic
RT   stem cell lines.";
RL   BMC Biol. 4:28-28(2006).
//
RX   PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113;
RA   Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R.,
RA   O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A.,
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RA   McDonald H., Asano J., Dhalla N., Ma K., Lee S., Ally A., Chahal N.,
RA   Menzies S., Siddiqui A., Holt R., Jones S., Gerhard D.S.,
RA   Thomson J.A., Eaves C.J., Marra M.A.;
RT   "LongSAGE profiling of nine human embryonic stem cell lines.";
RL   Genome Biol. 8:R113.1-R113.12(2007).
//
RX   PubMed=17572666; DOI=10.1038/nbt1318;
RA   Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W.,
RA   Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B.,
RA   Brooking J., Chen K.G., Choo A.B.H., Churchill G.A., Corbel M.,
RA   Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A.,
RA   Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S.,
RA   Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P.,
RA   Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K.,
RA   King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D.,
RA   Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M.,
RA   Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N.,
RA   O'Brien C.M., Oh S.K.W., Olsson C., Otonkoski T., Park K.-Y.,
RA   Passier R., Patel H., Patel M., Pedersen R., Pera M.F.,
RA   Piekarczyk M.S., Pera R.A.R., Reubinoff B.E., Robins A.J., Rossant J.,
RA   Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H.,
RA   Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T.,
RA   Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward D.,
RA   Weaver T.A., Young L.A., Zhang W.;
RT   "Characterization of human embryonic stem cell lines by the
RT   International Stem Cell Initiative.";
RL   Nat. Biotechnol. 25:803-816(2007).
//
RX   PubMed=21295703; DOI=10.1016/j.cell.2010.12.032;
RA   Bock C., Kiskinis E., Verstappen G., Gu H., Boulting G., Smith Z.D.,
RA   Ziller M., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K.,
RA   Meissner A.;
RT   "Reference maps of human ES and iPS cell variation enable
RT   high-throughput characterization of pluripotent cell lines.";
RL   Cell 144:439-452(2011).
//
RX   PubMed=21983960; DOI=10.1038/nmeth.1699;
RA   Phanstiel D.H., Brumbaugh J., Wenger C.D., Tian S., Probasco M.D.,
RA   Bailey D.J., Swaney D.L., Tervo M.A., Bolin J.M., Ruotti V.,
RA   Stewart R., Thomson J.A., Coon J.J.;
RT   "Proteomic and phosphoproteomic comparison of human ES and iPS
RT   cells.";
RL   Nat. Methods 8:821-827(2011).
//
RX   PubMed=22514597; DOI=10.1371/journal.pone.0030743;
RA   Sandt C., Feraud O., Oudrhiri N., Bonnet M.-L., Meunier M.-C.,
RA   Valogne Y., Bertrand A., Raphael M., Griscelli F., Turhan A.G.,
RA   Dumas P., Bennaceur-Griscelli A.;
RT   "Identification of spectral modifications occurring during
RT   reprogramming of somatic cells.";
RL   PLoS ONE 7:E30743-E30743(2012).
//
RX   PubMed=22802639; DOI=10.1073/pnas.1209979109;
RA   Kajiwara M., Aoi T., Okita K., Takahashi R., Inoue H., Takayama N.,
RA   Endo H., Eto K., Toguchida J., Uemoto S., Yamanaka S.;
RT   "Donor-dependent variations in hepatic differentiation from
RT   human-induced pluripotent stem cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12538-12543(2012).
//
RX   PubMed=23117585; DOI=10.1016/j.scr.2012.09.002;
RA   Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J.,
RA   Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.;
RT   "StemCellDB: the human pluripotent stem cell database at the National
RT   Institutes of Health.";
RL   Stem Cell Res. 10:57-66(2013).
//
RX   PubMed=24259714; DOI=10.1073/pnas.1319061110;
RA   Koyanagi-Aoi M., Ohnuki M., Takahashi K., Okita K., Noma H.,
RA   Sawamura Y., Teramoto I., Narita M., Sato Y., Ichisaka T., Amano N.,
RA   Watanabe A., Morizane A., Yamada Y., Sato T., Takahashi J.,
RA   Yamanaka S.;
RT   "Differentiation-defective phenotypes revealed by large-scale analyses
RT   of human pluripotent stem cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:20569-20574(2013).
//
RX   PubMed=27476965; DOI=10.1016/j.stem.2016.06.019;
RA   Nishizawa M., Chonabayashi K., Nomura M., Tanaka A., Nakamura M.,
RA   Inagaki A., Nishikawa M., Takei I., Oishi A., Tanabe K., Ohnuki M.,
RA   Yokota H., Koyanagi-Aoi M., Okita K., Watanabe A., Takaori-Kondo A.,
RA   Yamanaka S., Yoshida Y.;
RT   "Epigenetic variation between human induced pluripotent stem cell
RT   lines is an indicator of differentiation capacity.";
RL   Cell Stem Cell 19:341-354(2016).
//
RX   PubMed=28435121; DOI=10.1016/j.jprot.2017.04.017;
RA   Shekari F., Nezari H., Larijani M.R., Han C.-L., Baharvand H.,
RA   Chen Y.-J., Salekdeh G.H.;
RT   "Proteome analysis of human embryonic stem cells organelles.";
RL   J. Proteomics 162:108-118(2017).
//
RX   PubMed=28445466; DOI=10.1038/nature22312;
RA   Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C.,
RA   Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G.,
RA   Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A.,
RA   Eggan K.;
RT   "Human pluripotent stem cells recurrently acquire and expand dominant
RT   negative P53 mutations.";
RL   Nature 545:229-233(2017).
//
RX   PubMed=29246576; DOI=10.1016/j.scr.2017.08.004;
RA   Domingo-Reines J., Lopez-Ornelas A., Montes R., Romero T.,
RA   Rodriguez-Llamas J.L., Lara-Rodarte R., Gonzalez-Pozas F., Ayllon V.,
RA   Menendez P., Velasco I., Ramos-Mejia V.;
RT   "Hoxa9 and EGFP reporter expression in human embryonic stem cells
RT   (hESC) as useful tools for studying human development.";
RL   Stem Cell Res. 25:286-290(2017).
//
RX   PubMed=30388422; DOI=10.1016/j.stem.2018.10.009;
RA   Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S.,
RA   Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.;
RT   "20 years of human pluripotent stem cell research: it all started with
RT   five lines.";
RL   Cell Stem Cell 23:644-648(2018).
//