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Cellosaurus WA14 (CVCL_9775)

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Cell line name WA14
Synonyms WA 14; H14; GE14; WAe005-A; WICELLe005-A; WAe014-A
Accession CVCL_9775
Resource Identification Initiative To cite this cell line use: WA14 (RRID:CVCL_9775)
Comments Group: Clinical grade hESC cell line.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0064.
Registration: Swiss research registry; BAG-hES-IMP-0033.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-18.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Miscellaneous: Sibling to WA07 (CVCL_9772) and WA13 (CVCL_9774).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_U174 (NSC-H14)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): WiCell

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135815,16
D5S81811,13
D7S82010,11
D8S117913,14
D13S31711
D16S53911,13
D18S5112,14
D21S1130,31
FGA21
Penta D12,13
Penta E13,20
TH016,7
TPOX8
vWA15,16

Run an STR similarity search on this cell line
Web pages https://grants.nih.gov/stem_cells/registry/current.htm?id=416
https://stemcelldb.nih.gov/cellLineDetails.do?id=29
http://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M.
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Carpenter M.K., Rosler E.S., Fisk G.J., Brandenberger R., Ares X., Miura T., Lucero M., Rao M.S.
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R., Frane J.L., Crandall L.J., Daigh C.A., Conard K.R., Piekarczyk M.S., Llanas R.A., Thomson J.A.
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113
Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R., O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A., Morin R.D., Baross A., Zhao Y., Khattra J., Prabhu A.-L., Pandoh P., McDonald H., Asano J., Dhalla N., Ma K., Lee S., Ally A., Chahal N., Menzies S., Siddiqui A., Holt R., Jones S., Gerhard D.S., Thomson J.A., Eaves C.J., Marra M.A.
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M., Pedersen R., Pera M.F., Piekarczyk M.S., Pera R.A.R., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward D., Weaver T.A., Young L.A., Zhang W.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21983960; DOI=10.1038/nmeth.1699
Phanstiel D.H., Brumbaugh J., Wenger C.D., Tian S., Probasco M.D., Bailey D.J., Swaney D.L., Tervo M.A., Bolin J.M., Ruotti V., Stewart R., Thomson J.A., Coon J.J.
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002
Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J., Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=28445466; DOI=10.1038/nature22312
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S., Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections WiCell; wa14
WiCell; wa14-cgmp-material
WiCell; wa14-matched-research
Cell line databases/resources hPSCreg; WAe014-A
ISCR; 93
SKIP; SKIP001937
Biological sample resources BioSample; SAMN03471899
Gene expression databases GEO; GSM347925
GEO; GSM856733
Other Wikidata; Q54993517
Entry history
Entry creation06-Jun-2012
Last entry updated13-Nov-2018
Version number21