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Cellosaurus SEES3-1V human ELF5, clone5 (CVCL_A1I2)

[Text version]

Cell line name SEES3-1V human ELF5, clone5
Synonyms 1V-90551-1B
Accession CVCL_A1I2
Resource Identification Initiative To cite this cell line use: SEES3-1V human ELF5, clone5 (RRID:CVCL_A1I2)
Comments Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed.
Characteristics: Transfected with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a PiggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein.
Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Transfected with: HGNC; 3320; ELF5.
Transfected with: UniProtKB; P00722; E.coli lacZ.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P04483; E.coli Tn10 tetR.
Transfected with: UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (with del 1-360).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZJ91 (SEES3-1V)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=32433964; DOI=10.1016/j.celrep.2020.107655
Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M., Sakota M., Chikazawa N., Ookura C., Sato S., Ito N., Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K., Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-I., Oda M., Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O., Ko M.S.H.
Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.
Cell Rep. 31:107655-107655(2020)

Cross-references
Cell line collections RCB; HES0016
Other Wikidata; Q98130175
Entry history
Entry creation02-Jul-2020
Last entry update20-May-2021
Version number3