ID   SEES3-1V human EOMES, clone1
SY   1V-91688-4A
DR   RCB; HES0013
DR   Wikidata; Q98130211
RX   PubMed=32433964;
CC   Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed.
CC   Characteristics: Transfected with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a PiggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein.
CC   Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC   Transfected with: HGNC; 3372; EOMES.
CC   Transfected with: UniProtKB; P00722; Escherichia coli lacZ.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (with del 1-360).
OX   NCBI_TaxID=9606; ! Homo sapiens
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-07-20; Last updated: 23-06-22; Version: 4
RX   PubMed=32433964; DOI=10.1016/j.celrep.2020.107655;
RA   Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M.,
RA   Sakota M., Chikazawa N., Ookura C., Sato S., Ito N.,
RA   Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K.,
RA   Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-I., Oda M.,
RA   Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O.,
RA   Ko M.S.H.;
RT   "Generation and profiling of 2,135 human ESC lines for the systematic
RT   analyses of cell states perturbed by inducing single transcription
RT   factors.";
RL   Cell Rep. 31:107655.1-107655.23(2020).