ID   RUCDRi002-A-5
SY   TC-1133-CRISPRa
DR   hPSCreg; RUCDRi002-A-5
DR   Wikidata; Q102114853
RX   PubMed=33038615;
CC   From: University Medical Center Goettingen; Goettingen; Germany.
CC   Population: Hispanic.
CC   Characteristics: Using CRISPR/Cas9 two constructs one containing CAGpromoter-dCas9-VPR-2A-tdTomato-gGH and the other EF1alpha-promoter-Pac-SV40polyA were introduced in the AAVS1 safe harbor locus (PubMed=33038615).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Derived from sampling site: Umbilical cord blood.
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_RH35 ! ND50038
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 29-10-20; Last updated: 23-06-22; Version: 4
RX   PubMed=33038615; DOI=10.1016/j.scr.2020.101944;
RA   Schoger E., Argyriou L., Zimmermann W.-H., Cyganek L., Zelarayan L.C.;
RT   "Generation of homozygous CRISPRa human induced pluripotent stem cell
RT   (hiPSC) lines for sustained endogenous gene activation.";
RL   Stem Cell Res. 48:101944-101944(2020).