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Cellosaurus NH32 (CVCL_A1RF)

[Text version]
Cell line name NH32
Accession CVCL_A1RF
Resource Identification Initiative To cite this cell line use: NH32 (RRID:CVCL_A1RF)
Comments Population: Caucasian.
Knockout cell: Method=Promoterless gene targeting; HGNC; 11998; TP53.
Transfected with: UniProtKB; P06988; Escherichia coli hisD.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
Sequence variations
  • Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=26774668).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0561 (TK6)
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
Publications

PubMed=10397247
Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.
Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.
Cancer Res. 59:3073-3076(1999)

PubMed=22042714; DOI=10.1093/mutage/ger074
Hashimoto K., Nakajima Y., Uematsu R., Chatani F.
Difference in susceptibility to morphological changes in the nucleus to aneugens between p53-competent and p53-abrogated lymphoblastoid cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.
Mutagenesis 27:287-293(2012)

PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006
Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B., Dobrovolsky V.N.
Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.
Mutat. Res. 795:60-69(2016)

Cross-references
Encyclopedic resources Wikidata; Q105510331
Entry history
Entry creation12-Jan-2021
Last entry update29-Jun-2023
Version number4