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Cellosaurus 2fTGH-U1A (CVCL_A3FC)

[Text version]

Cell line name 2fTGH-U1A
Synonyms U1A; 11,1
Accession CVCL_A3FC
Resource Identification Initiative To cite this cell line use: 2fTGH-U1A (RRID:CVCL_A3FC)
Comments Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Transfected with: UniProtKB; P0A9M5; E.coli gpt.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Sequence variations Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0115 (2fTGH)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line

PubMed=2513475; DOI=10.1128/MCB.9.11.4605
Pellegrini S., John J., Shearer M., Kerr I.M., Stark G.R.
Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.
Mol. Cell. Biol. 9:4605-4612(1989)

Other Wikidata; Q105505902
Entry history
Entry creation12-Jan-2021
Last entry update20-May-2021
Version number2