Home  |  Contact

Cellosaurus MCF7 dCas9-KRAB (CVCL_A4CH)

[Text version]
Cell line name MCF7 dCas9-KRAB
Accession CVCL_A4CH
Resource Identification Initiative To cite this cell line use: MCF7 dCas9-KRAB (RRID:CVCL_A4CH)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a construct containing KRAB-dCas9-2A-hph-2A-RFP-PA was introduced in the AAVS1 safe harbor locus.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO10
D5S81811,12
D7S8208,9
D13S31711
D16S53911,12
TH016
TPOX9,12
vWA14,15

Run an STR similarity search on this cell line
Cross-references
Cell line collections (Providers) ATCC; HTB-22dCas9-KRAB
Cell line databases/resources cancercelllines; CVCL_A4CH
Encyclopedic resources Wikidata; Q105510079
Entry history
Entry creation12-Jan-2021
Last entry update30-Jan-2024
Version number7