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Cellosaurus WAe001-A-47 (CVCL_A4TX)

[Text version]
Cell line name WAe001-A-47
Synonyms H1-hVGLL4-Tet-On; WMUe002-A-47
Accession CVCL_A4TX
Resource Identification Initiative To cite this cell line use: WAe001-A-47 (RRID:CVCL_A4TX)
Comments From: Wenzhou Medical University; Wenzhou; China.
Characteristics: Dox-inducible expression of VGLL4. The cell has been transfected with a PB-TRE6P-VGLL4-P2A-EGFP-3Flag-PA-EF1a-5'LTR-rtTA-T2A-puroR construct (PubMed=33385792).
Transfected with: HGNC; 28966; VGLL4.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33385792; DOI=10.1016/j.scr.2020.102142
Quan Y.-Y., Zhang T.-C., Zhang H., Yang J.-W., Jin P.-F., Ma J.-S., Fan Y.-Y., Fan J.-M., Fan X.-F., Gong Y.-S., Li M., Wang Y.-Y.
Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system.
Stem Cell Res. 50:102142-102142(2021)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-47
Encyclopedic resources Wikidata; Q107117407
Entry history
Entry creation20-May-2021
Last entry update29-Jun-2023
Version number6