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Cellosaurus H1.CD43/CD144DR (CVCL_A4WB)

[Text version]

Cell line name H1.CD43/CD144DR
Synonyms H1 VEC-tdTomato/CD43-eGFP dual reporter
Accession CVCL_A4WB
Resource Identification Initiative To cite this cell line use: H1.CD43/CD144DR (RRID:CVCL_A4WB)
Comments From: University of Wisconsin; Madison; USA.
Characteristics: Using ZFN, a 2A-PuroR-CD43_promoter-eGFP was introduced in the AAVS1 safe harbor locus and CDH5(CD144) promoter-tdTomato-Zeo construct was transferred using a PiggyBac transposon vector (PubMed=27879215).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: tdTomato; a bright red fluorescent protein derived from drFP583 (DsRed).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P17493; S.hindustanus ble.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=27879215

Markers:
AmelogeninX,Y
CSF1PO12,13
D3S135815
D5S8189,11
D7S8208,12
D8S117912,13
D13S3178,11
D16S5399,13
D18S5117,18
D21S1128,32.2
FGA20,24
Penta D10,13
Penta E10,12
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=27879215; DOI=10.1016/j.scr.2016.09.004
Jung H.S., Uenishi G., Kumar A., Park M.A., Raymond M., Fink D., McLeod E., Slukvin I.
A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition.
Stem Cell Res. 17:401-405(2016)

Cross-references
Other Wikidata; Q107115213
Entry history
Entry creation20-May-2021
Last entry update23-Sep-2021
Version number2