ID   WAe001-A-80
AC   CVCL_A4WB
SY   H1.CD43/CD144DR; H1 VEC-tdTomato/CD43-eGFP dual reporter; WISCe011-A-80
DR   hPSCreg; WAe001-A-80
DR   Wikidata; Q107115213
RX   PubMed=27879215;
CC   From: University of Wisconsin; Madison; USA.
CC   Characteristics: Using ZFN a 2A-PuroR-CD43_promoter-eGFP was introduced in the AAVS1 safe harbor locus and CDH5(CD144) promoter-tdTomato-Zeo construct was transferred using a PiggyBac transposon vector (PubMed=27879215).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
ST   Source(s): PubMed=27879215
ST   Amelogenin: X,Y
ST   CSF1PO: 12,13
ST   D13S317: 8,11
ST   D16S539: 9,13
ST   D18S51: 17,18
ST   D21S11: 28,32.2
ST   D3S1358: 15
ST   D5S818: 9,11
ST   D7S820: 8,12
ST   D8S1179: 12,13
ST   FGA: 20,24
ST   Penta D: 10,13
ST   Penta E: 10,12
ST   TH01: 9.3
ST   TPOX: 8,11
ST   vWA: 15,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 20-05-21; Last updated: 29-06-23; Version: 6
//
RX   PubMed=27879215; DOI=10.1016/j.scr.2016.09.004;
RA   Jung H.S., Uenishi G., Kumar A., Park M.A., Raymond M., Fink D.,
RA   McLeod E., Slukvin I.I.;
RT   "A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual
RT   reporter cell line for study endothelial to hematopoietic
RT   transition.";
RL   Stem Cell Res. 17:401-405(2016).
//