Cellosaurus NCI-H249 (CVCL_A537)

Cell line name NCI-H249
Synonyms H249; H-249; NCIH249
Accession CVCL_A537
Resource Identification Initiative To cite this cell line use: NCI-H249 (RRID:CVCL_A537)
Comments Problematic cell line: Contaminated. Shown to be a NCI-H69 derivative (PubMed=20143388).
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Small cell lung carcinoma (NCIt: C4917)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1579 (NCI-H69)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO10,12
D13S31712
D16S53911
D5S81811,13
D7S8209
TH018,9
TPOX10
vWA16,17
Web pages http://www.atcc.org/support/faqs/4bf60/NCIH249%20ATCC%20CRL5827-1073.aspx
http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf
Publications

PubMed=6148444; DOI=10.1093/jnci/73.4.801
Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H., Mitchell J.B.
Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=2985257
Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. 63 Suppl. 24:210-217(1996)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Modi S., Kubo A., Oie H., Coxon A.B., Rehmatulla A., Kaye F.J.
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections ATCC; CRL-5827 - Discontinued
KCLB; 90249 - Discontinued
Biological sample resources BioSample; SAMN03151645
BioSample; SAMN03151860
BioSample; SAMN03471746
Gene expression databases GEO; GSM845545
GEO; GSM844646
Polymorphism and mutation databases Cosmic; 755460
Cosmic; 877204
Cosmic; 980931
Cosmic; 1006529
Cosmic; 1021857
Cosmic; 1032397
Cosmic; 2125249