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Cellosaurus HepG2.105 (CVCL_A5AW)

[Text version]

Cell line name HepG2.105
Synonyms HepG2.TA2-7.105
Accession CVCL_A5AW
Resource Identification Initiative To cite this cell line use: HepG2.105 (RRID:CVCL_A5AW)
Comments Population: Caucasian.
Characteristics: Expresses an HBV genome (subtype AYW) under the control of a tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. Transcription of HBV pre-genomic RNA is induced upon doxycycline removal from the culture medium, leading to capsid assembly and DNA synthesis (PubMed=16935386).
Transfected with: UniProtKB; P04483; E.coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with del 1-360).
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from sampling site: Liver.
Sequence variations Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=16935386; DOI=10.1016/j.jhep.2006.05.019
Sun D.-X., Nassal M.
Stable HepG2- and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus.
J. Hepatol. 45:636-645(2006)

Cross-references
Other Wikidata; Q107115331
Entry history
Entry creation20-May-2021
Last entry update23-Sep-2021
Version number2