ID   HepG2.105
SY   HepG2.TA2-7.105
DR   Wikidata; Q107115331
RX   PubMed=16935386;
CC   Population: Caucasian.
CC   Characteristics: Expresses an HBV genome (subtype AYW) under the control of a tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. Transcription of HBV pre-genomic RNA is induced upon doxycycline removal from the culture medium, leading to capsid assembly and DNA synthesis (PubMed=16935386).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Transfected with: UniProtKB; P04483; E.coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with del 1-360).
CC   Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from sampling site: Liver.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0027 ! Hep-G2
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 23-09-21; Version: 2
RX   PubMed=16935386; DOI=10.1016/j.jhep.2006.05.019;
RA   Sun D.-X., Nassal M.;
RT   "Stable HepG2- and Huh7-based human hepatoma cell lines for efficient
RT   regulated expression of infectious hepatitis B virus.";
RL   J. Hepatol. 45:636-645(2006).