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Cellosaurus HEK293S GnTI- (CVCL_A785)

[Text version]
Cell line name HEK293S GnTI-
Synonyms 293SGnTI(-); HEK293SG; 293SG
Accession CVCL_A785
Resource Identification Initiative To cite this cell line use: HEK293S GnTI- (RRID:CVCL_A785)
Comments Characteristics: Lacks N-acetylglucosaminyltransferase I activity and accordingly predominantly modifies glycoproteins with the Man5GlcNAc2 N-glycan. Used for the production of homogenously N-glycosylated proteins (PubMed=25182477).
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A784 (HEK293S)
Children:
CVCL_6E38 (293SGlycoDelete)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11,12
D2S133819
D3S135815,17
D5S8188,9
D7S82011,12
D8S117912,14
D13S31712,14
D16S5399
D18S5118
D19S43318
D21S1128,30.2
FGA23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX11
vWA16,19

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Publications

PubMed=12370423; DOI=10.1073/pnas.212519299
Reeves P.J., Callewaert N., Contreras R., Khorana H.G.
Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.
Proc. Natl. Acad. Sci. U.S.A. 99:13419-13424(2002)

PubMed=25182477; DOI=10.1038/ncomms5767
Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767.1-4767.12(2014)

DOI=10.13005/bpj/1414
Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-3022
Encyclopedic resources Wikidata; Q54882485
Entry history
Entry creation06-Jun-2012
Last entry update05-Oct-2023
Version number13