ID   RUCDRi002-A-15
AC   CVCL_A7LR
SY   TC-1133-CRISPRa2; hiPSC-CRISPRa2
DR   hPSCreg; RUCDRi002-A-15
DR   Wikidata; Q107116790
RX   PubMed=34481190;
WW   https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=478
CC   From: University Medical Center Goettingen; Goettingen; Germany.
CC   Population: Hispanic.
CC   Characteristics: Using CRISPR/Cas9 a dCas9-VPR-2A-tdTomato-WPRE construct containing dCas9 fused to heterotrimeric VPR transactivator consisting of VP64, p65, and RTA domains along with a tdTomato and a woodchuck hepatitis post-transcriptional regulatory element (WPRE) was introduced in the AAVS1 safe harbor locus (PubMed=34481190).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: tdTomato; a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Derived from sampling site: Umbilical cord blood.
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_RH35 ! ND50038
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 20-05-21; Last updated: 23-09-21; Version: 2
//
RX   PubMed=34481190; DOI=10.1016/j.scr.2021.102518;
RA   Schoger E., Zimmermann W.H., Cyganek L., Zelarayan L.C.;
RT   "Establishment of a second generation homozygous CRISPRa human induced
RT   pluripotent stem cell (hiPSC) line for enhanced levels of endogenous
RT   gene activation.";
RL   Stem Cell Res. 56:102518-102518(2021).
//