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Cellosaurus RUCDRi002-A-16 (CVCL_A7LS)

[Text version]

Cell line name RUCDRi002-A-16
Synonyms TC-1133-CRISPRi
Accession CVCL_A7LS
Resource Identification Initiative To cite this cell line use: RUCDRi002-A-16 (RRID:CVCL_A7LS)
Comments From: University Medical Center Goettingen; Goettingen; Germany.
Population: Hispanic.
Characteristics: Using CRISPR/Cas9 a CAG-dCas9-KRAB-T2A-tdTomato-EF1alpha-puroR construct was introduced in the AAVS1 safe harbor locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: tdTomato; a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from sampling site: Umbilical cord blood.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RH35 (ND50038)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Web pages https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=479
Publications

PubMed=34343828; DOI=10.1016/j.scr.2021.102473
Schoger E., Zimmermann W.H., Cyganek L., Zelarayan L.C.
Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression.
Stem Cell Res. 55:102473-102473(2021)

Cross-references
Cell line databases/resources hPSCreg; RUCDRi002-A-16
Other Wikidata; Q107116791
Entry history
Entry creation20-May-2021
Last entry update23-Sep-2021
Version number2