|Cell line name||A549-Dual hACE2-TMPRSS2|
|Resource Identification Initiative||To cite this cell line use: A549-Dual hACE2-TMPRSS2 (RRID:CVCL_A7ZQ)|
|Comments||Group: SARS-CoV2 research cell line.|
Characteristics: Ideal for studying SARS-CoV-2 viral entry into host cells, the viral RNA sensing pathway, as well as screening small molecule inhibitors and neutralizing antibodies. These cells are permissive to infection by SARS-CoV-2 Spike-pseudotyped viral particles (InVivoGen).
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
Transfected with: HGNC; 13557; ACE2.
Transfected with: HGNC; 11876; TMPRSS2.
Transfected with: HGNC; 439; ALPP.
Transfected with: Lucia luciferase; a synthetic gene designed on natural secreted luciferase genes from marine copepods.
Transfected with: UniProtKB; P17493; S.hindustanus ble.
Transfected with: UniProtKB; P33967; B.cereus Bsr.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from sampling site: Lung.
|Sequence variations||Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).|
|Disease||Lung adenocarcinoma (NCIt: C3512)|
|Species of origin||Homo sapiens (Human)
(NCBI Taxonomy: 9606)
|Hierarchy||Parent: CVCL_5I73 (A549-Dual)|
|Sex of cell||Male|
|Age at sampling||58Y|
|Category||Cancer cell line|
|Last entry update||20-May-2021|