ID   A549-Dual KO-MDA5 hACE2-TMPRSS2
AC   CVCL_A7ZR
DR   cancercelllines; CVCL_A7ZR
DR   Wikidata; Q107113330
WW   https://www.invivogen.com/a549dual-hace2tmprss2-cells
CC   Group: SARS-CoV-2 research cell line.
CC   Population: Caucasian.
CC   Characteristics: Ideal for studying SARS-CoV-2 viral entry into host cells, the viral RNA sensing pathway, as well as screening small molecule inhibitors and neutralizing antibodies. These cells are permissive to infection by SARS-CoV-2 Spike-pseudotyped viral particles (InvivoGen).
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
CC   Knockout cell: Method=Not specified; HGNC; 18873; IFIH1.
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: HGNC; 13557; ACE2.
CC   Transfected with: HGNC; 11876; TMPRSS2.
CC   Transfected with: HGNC; 439; ALPP.
CC   Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine copepods.
CC   Transfected with: UniProtKB; P33967; B.cereus Bsr.
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A7ZP ! A549-Dual KO-MDA5
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 30-01-24; Version: 8
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