Home  |  Contact

Cellosaurus A549-Dual KO-RIG-I hACE2-TMPRSS2 (CVCL_A7ZS)

[Text version]

Cell line name A549-Dual KO-RIG-I hACE2-TMPRSS2
Accession CVCL_A7ZS
Resource Identification Initiative To cite this cell line use: A549-Dual KO-RIG-I hACE2-TMPRSS2 (RRID:CVCL_A7ZS)
Comments Group: SARS-CoV2 research cell line.
Population: Caucasian.
Characteristics: Ideal for studying SARS-CoV-2 viral entry into host cells, the viral RNA sensing pathway, as well as screening small molecule inhibitors and neutralizing antibodies. These cells are permissive to infection by SARS-CoV-2 Spike-pseudotyped viral particles (InVivoGen).
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
Knockout cell: Method=Not specified; HGNC; 19102; DDX58.
Transfected with: HGNC; 13557; ACE2.
Transfected with: HGNC; 11876; TMPRSS2.
Transfected with: HGNC; 439; ALPP.
Transfected with: Lucia luciferase; a synthetic gene designed on natural secreted luciferase genes from marine copepods.
Transfected with: UniProtKB; P17493; S.hindustanus ble.
Transfected with: UniProtKB; P33967; B.cereus Bsr.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from sampling site: Lung.
Sequence variations Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A7ZN (A549-Dual KO-RIG-I)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Web pages https://www.invivogen.com/a549dual-hace2tmprss2-cells
Entry history
Entry creation20-May-2021
Last entry update20-May-2021
Version number1