| Cell line name | THP1-Difluo hLC3 |
|---|---|
| Accession | CVCL_A8BZ |
| Resource Identification Initiative | To cite this cell line use: THP1-Difluo hLC3 (RRID:CVCL_A8BZ) |
| Comments | Population: Japanese. Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen). Characteristics: Stably expresses a RFP-GFP-MAP1LC3B construct. Transfected with: HGNC; 13352; MAP1LC3B. Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. |
| Sequence variations |
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| Disease | Childhood acute monocytic leukemia (NCIt: C9163) Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_X601 (THP1-Null) |
| Sex of cell | Male |
| Age at sampling | 1Y |
| Category | Cancer cell line |
| Web pages | https://www.invivogen.com/thp1-difluo-hlc3 |
| Cross-references | |
| Cell line databases/resources | cancercelllines; CVCL_A8BZ |
| Encyclopedic resources | Wikidata; Q107117044 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 30-Jan-2024 |
| Version number | 6 |