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Cellosaurus HVRDe008-A-1 (CVCL_A8ZA)

[Text version]

Cell line name HVRDe008-A-1
Synonyms GAPluc
Accession CVCL_A8ZA
Resource Identification Initiative To cite this cell line use: HVRDe008-A-1 (RRID:CVCL_A8ZA)
Comments Characteristics: Using CRISPR/Cas9, a P2A-Luc2-IRES-Puro-pA construct was inserted at the 3'end of one allele of GAPDH (PubMed=34088000).
Transfected with: UniProtKB; P08659; Firefly luciferase (with optimized codon usage for mammalian expression).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B207 (HUES 8)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=34088000; DOI=10.1016/j.scr.2021.102371
Gerace D., Boulanger K.R., Kenty J.H.-R., Melton D.A.
Generation of a heterozygous GAPDH-luciferase human ESC line (HVRDe008-A-1) for in vivo monitoring of stem cells and their differentiated progeny.
Stem Cell Res. 53:102371-102371(2021)

Cross-references
Cell line databases/resources hPSCreg; HVRDe008-A-1
Biological sample resources BioSamples; SAMEA8633544
Encyclopedic resources Wikidata; Q108820515
Entry history
Entry creation23-Sep-2021
Last entry update16-Dec-2021
Version number2