ID   HVRDe008-A-1
AC   CVCL_A8ZA
SY   GAPluc; MLBe009-A-1
DR   BioSamples; SAMEA8633544
DR   hPSCreg; HVRDe008-A-1
DR   Wikidata; Q108820515
RX   PubMed=34088000;
CC   Characteristics: Using CRISPR/Cas9 a P2A-Luc2-IRES-PuroR-pA construct was integrated at the 3'end of one allele of GAPDH (PubMed=34088000).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase (with optimized codon usage for mammalian expression).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B207 ! HUES 8
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 23-09-21; Last updated: 05-10-23; Version: 7
//
RX   PubMed=34088000; DOI=10.1016/j.scr.2021.102371;
RA   Gerace D., Boulanger K.R., Kenty J.H.-R., Melton D.A.;
RT   "Generation of a heterozygous GAPDH-luciferase human ESC line
RT   (HVRDe008-A-1) for in vivo monitoring of stem cells and their
RT   differentiated progeny.";
RL   Stem Cell Res. 53:102371-102371(2021).
//