ID   LUHMES
AC   CVCL_B056
SY   LUnd Human MESencephalic
DR   ABM; T0284
DR   ATCC; CRL-2927
DR   BioSample; SAMN03471701
DR   BioSample; SAMN08723614
DR   Wikidata; Q54903259
RX   PubMed=16000623;
RX   PubMed=21434924;
RX   PubMed=24173167;
RX   PubMed=30602607;
RX   PubMed=32150624;
WW   https://www.atcc.org/en/support/technical-support/faqs/growth-and-morphology-of-luhmes-crl-2927
CC   Characteristics: Embryonic neuronal precursor cells that can be maintained as proliferating cells due to the expression of a tetracycline-regulatable (Tet-Off) v-myc transgene. Can be differentiated to post-mitotic neurons by the addition of tetracycline, GDNF and dibutyryl cAMP.
CC   Transfected with: UniProtKB; P01110; Avian myelocytomatosis virus MC29 v-Myc.
CC   Transformant: v-Myc.
CC   Derived from site: In situ; Fetal brain, ventral mesencephalon; UBERON=UBERON_0001943.
ST   Source(s): ATCC
ST   Amelogenin: X
ST   CSF1PO: 13,14
ST   D13S317: 9,11
ST   D16S539: 11,12
ST   D18S51: 12
ST   D19S433: 14,15
ST   D21S11: 30,31
ST   D2S1338: 17,24
ST   D3S1358: 17,18
ST   D5S818: 11,13
ST   D7S820: 11,13
ST   D8S1179: 12,13
ST   FGA: 19,21
ST   Penta D: 12,13
ST   Penta E: 11,13
ST   TH01: 7,9.3
ST   TPOX: 8
ST   vWA: 14,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B061 ! MESC2.10
SX   Female
AG   8FW
CA   Conditionally immortalized cell line
DT   Created: 06-06-12; Last updated: 05-10-23; Version: 16
//
RX   PubMed=16000623; DOI=10.1523/JNEUROSCI.1746-05.2005;
RA   Lotharius J., Falsig J., van Beek J., Payne S., Dringen R.,
RA   Brundin P., Leist M.;
RT   "Progressive degeneration of human mesencephalic neuron-derived cells
RT   triggered by dopamine-dependent oxidative stress is dependent on the
RT   mixed-lineage kinase pathway.";
RL   J. Neurosci. 25:6329-6342(2005).
//
RX   PubMed=21434924; DOI=10.1111/j.1471-4159.2011.07255.x;
RA   Scholz D., Poltl D., Genewsky A., Weng M., Waldmann T.,
RA   Schildknecht S., Leist M.;
RT   "Rapid, complete and large-scale generation of post-mitotic neurons
RT   from the human LUHMES cell line.";
RL   J. Neurochem. 119:957-971(2011).
//
RX   PubMed=24173167; DOI=10.14573/altex.2013.4.427;
RA   Schildknecht S., Karreman C., Poltl D., Efremova L., Kullmann C.,
RA   Gutbier S., Krug A., Scholz D., Gerding H.R., Leist M.;
RT   "Generation of genetically-modified human differentiated cells for
RT   toxicological tests and the study of neurodegenerative diseases.";
RL   ALTEX 30:427-444(2013).
//
RX   PubMed=30602607; DOI=10.1128/JVI.02210-18;
RA   Edwards T.G., Bloom D.C.;
RT   "Lund human mesencephalic (LUHMES) neuronal cell line supports HSV-1
RT   latency in vitro.";
RL   J. Virol. 93:e02210.18.1-e02210.18.14(2019).
//
RX   PubMed=32150624; DOI=10.14573/altex.1911111;
RA   Brull M., Spreng A.-S., Gutbier S., Loser D., Krebs A., Reich M.,
RA   Kraushaar U., Britschgi M., Patsch C., Leist M.;
RT   "Incorporation of stem cell-derived astrocytes into neuronal organoids
RT   to allow neuro-glial interactions in toxicological studies.";
RL   ALTEX 37:409-428(2020).
//