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Cellosaurus ART [Human OVCAR-8 contaminated cell line] (CVCL_B0CC)

[Text version]
Cell line name ART [Human OVCAR-8 contaminated cell line]
Accession CVCL_B0CC
Resource Identification Initiative To cite this cell line use: ART [Human OVCAR-8 contaminated cell line] (RRID:CVCL_B0CC)
Comments Problematic cell line: Contaminated. Parent cell line (NCI-ADR-RES) has been shown to be a OVCAR-8 derivative.
Population: Caucasian.
Characteristics: Engineered to overexpress the SV40 large T antigen.
Virology: Can be used to evaluate the infectious entry of polyomaviruses (BKPyV, JCPyV, SV40, MCPyV) and human papillomaviruses. Pathogenic variants of the JCPyV virus previously believed to be non-infectious can infect this cell line (ATCC=CRL-3468).
Doubling time: ~34 hours (ATCC=CRL-3468).
Transfected with: UniProtKB; P03070; SV40 large T antigen.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Sequence variations
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Gln26Arg (c.77A>G); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3430; ERBB2; Simple; p.Gly776Val (c.2327G>T); ClinVar=VCV000163410; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Pro121His (c.362C>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; c.376-1G>A (p.Tyr126_Lys132del, c.376_396del21); ClinVar=VCV000481003; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
Disease High grade ovarian serous adenocarcinoma (NCIt: C105555)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1629 (OVCAR-8)
Sex of cell Female
Age at sampling 64Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11
D5S81812
D7S82020
D13S31712
D16S53913
TH017
TPOX8
vWA16,17

Run an STR similarity search on this cell line
Publications

PubMed=23843634; DOI=10.1128/JVI.01189-13
Pastrana D.V., Ray U., Magaldi T.G., Schowalter R.M., Cuburu N., Buck C.B.
BK polyomavirus genotypes represent distinct serotypes with distinct entry tropism.
J. Virol. 87:10105-10113(2013)

PubMed=29091757; DOI=10.1016/j.celrep.2017.10.027
Geoghegan E.M., Pastrana D.V., Schowalter R.M., Ray U., Gao W., Ho M., Pauly G.T., Sigano D.M., Kaynor C., Cahir-McFarland E., Combaluzier B., Grimm J., Buck C.B.
Infectious entry and neutralization of pathogenic JC polyomaviruses.
Cell Rep. 21:1169-1179(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-3468
Cell line databases/resources cancercelllines; CVCL_B0CC
Encyclopedic resources Wikidata; Q108819855
Entry history
Entry creation23-Sep-2021
Last entry update05-Oct-2023
Version number7