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Cellosaurus HUES 2 (CVCL_B150)

[Text version]
Cell line name HUES 2
Synonyms HUES-2; HUES2; HuES2; HVRDe002-A
Accession CVCL_B150
Resource Identification Initiative To cite this cell line use: HUES 2 (RRID:CVCL_B150)
Comments Problematic cell line: Misidentified. Presence of a Y chromosome in cell line that was thought to be of female origin.
From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0015.
Registration: UK Stem Cell Bank (UKSCB); R-06-006, Steering comm. appl. SCSC04-25(2).
Doubling time: 24 hours (PubMed=14999088).
Omics: DNA methylation analysis.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798; PubMed=21637780

Markers:
AmelogeninX,Y
CSF1PO12
D3S135814,18
D5S81811,12
D7S8209,13
D8S11798,12
D13S31711,12
D16S53911,13
D18S5112,16
D21S1131,32
FGA18,23
Penta D11,13
Penta E7,15
TH015,6
TPOX9,11
vWA14,16

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Publications

PubMed=14999088; DOI=10.1056/NEJMsr040330
Cowan C.A., Klimanskaya I.V., McMahon J., Atienza J., Witmyer J., Zucker J.P., Wang S.-P., Morton C.C., McMahon A.P., Powers D., Melton D.A.
Derivation of embryonic stem-cell lines from human blastocysts.
N. Engl. J. Med. 350:1353-1356(2004)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.-H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.-W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M.J., Pedersen R., Pera M.F., Piekarczyk M.S., Reijo Pera R.A., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T.T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward-van Oostwaard D., Weaver T.A., Young L.A., Zhang W.-D.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001
Chen A.E., Egli D., Niakan K.K., Deng J., Akutsu H., Yamaki M., Cowan C.A., Fitz-Gerald C., Zhang K., Melton D.A., Eggan K.C.
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

Cross-references
Cell line databases/resources hPSCreg; HVRDe002-A
ISCR; 103
NIHhESC; NIHhESC-10-0015
SKIP; SKIP001480
SKIP; SKIP001906
Encyclopedic resources Wikidata; Q54896750
Gene expression databases GEO; GSM608010
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number18