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Cellosaurus HUES 3 (CVCL_B161)

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Cell line name HUES 3
Synonyms HUES-3; HuES-3; HUES3; HuES3; Hues3; HVRDe003-A
Accession CVCL_B161
Resource Identification Initiative To cite this cell line use: HUES 3 (RRID:CVCL_B161)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0016.
Registration: Swiss research registry; BAG-hES-IMP-0036.
Registration: UK Stem Cell Bank (UKSCB); R-05-034, Steering comm. appl. SCSC04-25(3).
Doubling time: 60 hours (PubMed=14999088).
Omics: DNA methylation analysis.
Omics: Transcriptome analysis.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_X724 (HUES 3 Hb9::GFP)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798; PubMed=20973834; PubMed=21637780

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135815,16
D5S81812
D7S8208,11
D8S117912,13
D13S3178,14
D16S53912,14
D18S5113,17
D21S1128,29
FGA22,24
Penta D9,12
Penta E11,12
TH017,8
TPOX8,11
vWA16,17

Run an STR similarity search on this cell line
Web pages https://grants.nih.gov/stem_cells/registry/current.htm?id=34
Publications

PubMed=14999088; DOI=10.1056/NEJMsr040330
Cowan C.A., Klimanskaya I., McMahon J., Atienza J., Witmyer J., Zucker J.P., Wang S., Morton C.C., McMahon A.P., Powers D., Melton D.A.
Derivation of embryonic stem-cell lines from human blastocysts.
N. Engl. J. Med. 350:1353-1356(2004)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M., Pedersen R., Pera M.F., Piekarczyk M.S., Pera R.A.R., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward D., Weaver T.A., Young L.A., Zhang W.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001
Chen A.E., Egli D., Niakan K.K., Deng J., Akutsu H., Yamaki M., Cowan C., Fitz-Gerald C., Zhang K., Melton D.A., Eggan K.
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=20973834; DOI=10.1111/j.1749-0774.2010.00090.x
Wu R.-R., Xu C.-M., Jin F., Tan Z., Gu B., Chen L.-B., Yao X., Zhang M.
Derivation, characterization and differentiation of a new human embryonic stem cell line from a Chinese hatched blastocyst assisted by a non-contact laser system.
Hum. Cell 23:89-102(2010)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032
Bock C., Kiskinis E., Verstappen G., Gu H., Boulting G., Smith Z.D., Ziller M., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

PubMed=22514597; DOI=10.1371/journal.pone.0030743
Sandt C., Feraud O., Oudrhiri N., Bonnet M.-L., Meunier M.-C., Valogne Y., Bertrand A., Raphael M., Griscelli F., Turhan A.G., Dumas P., Bennaceur-Griscelli A.
Identification of spectral modifications occurring during reprogramming of somatic cells.
PLoS ONE 7:E30743-E30743(2012)

Cross-references
Cell line databases/resources hPSCreg; HVRDe003-A
ISCR; 104
SKIP; SKIP001481
SKIP; SKIP001907
Ontologies CLO; CLO_0037316
EFO; EFO_0007080
Biological sample resources eagle-i; harvard.eagle-i.net/i/00000140-2fd4-40c4-a870-ad6980000000
ENCODE; ENCBS433HCT
Gene expression databases GEO; GSM608011
GEO; GSM608029
GEO; GSM621767
GEO; GSM637759
GEO; GSM670075
Other Wikidata; Q54896761
Entry history
Entry creation06-Jun-2012
Last entry updated05-Jul-2019
Version number18