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Cellosaurus HUES 64 (CVCL_B199)

Cell line name HUES 64
Synonyms HUES64; HuES64
Accession CVCL_B199
Resource Identification Initiative To cite this cell line use: HUES 64 (RRID:CVCL_B199)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0067.
Registration: Swiss research registry; BAG-hES-IMP-0054.
Omics: Deep exome analysis.
Omics: Genome sequenced.
Omics: H3K27ac ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: H3K9me3 ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Donor information: Embryo is sibling to those giving rise to HUES 62 (Cellosaurus=CVCL_B197) and HUES 63 (Cellosaurus=CVCL_B198).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798

Markers:
AmelogeninX,Y
CSF1PO12
D2S133818,20
D3S135815
D5S81811,13
D7S82011,12
D8S117912,14
D16S53911,13
D19S43313,14
D21S1131,31.2
TH016,9
TPOX8,10
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001
Chen A.E., Egli D., Niakan K.K., Deng J., Akutsu H., Yamaki M., Cowan C.A., Fitz-Gerald C., Zhang K., Melton D.A., Eggan K.C.
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032
Bock C., Kiskinis E., Verstappen G., Gu H.-C., Boulting G.L., Smith Z.D., Ziller M.J., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K.C., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=28445466; DOI=10.1038/nature22312
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.C.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.
Nature 545:229-233(2017)

Cross-references
Cell line databases/resources ISCR; 790
NIHhESC; NIHhESC-10-0067
SKIP; SKIP001551
Biological sample resources ENCODE; ENCBS015UPH
ENCODE; ENCBS078ITI
ENCODE; ENCBS179VGA
ENCODE; ENCBS183DGX
ENCODE; ENCBS389KQW
ENCODE; ENCBS479IFR
ENCODE; ENCBS553VXQ
ENCODE; ENCBS614QHK
ENCODE; ENCBS666HUG
ENCODE; ENCBS766XXZ
ENCODE; ENCBS918DAB
Encyclopedic resources Wikidata; Q54896803
Experimental variables resources EFO; EFO_0007089
Gene expression databases GEO; GSM621594
GEO; GSM637772
GEO; GSM669928
GEO; GSM669932
GEO; GSM669963
GEO; GSM669966
GEO; GSM669967
GEO; GSM669974
GEO; GSM772749
GEO; GSM772750
GEO; GSM772751
GEO; GSM772752
GEO; GSM772754
GEO; GSM772756
GEO; GSM772800
GEO; GSM772807
GEO; GSM772856
GEO; GSM772912
GEO; GSM772913
GEO; GSM772971
GEO; GSM772977
GEO; GSM772978
GEO; GSM772979
GEO; GSM772980
GEO; GSM773002
GEO; GSM773003
GEO; GSM997249
GEO; GSM1112775
GEO; GSM1112834
GEO; GSM1112837
GEO; GSM1112840
GEO; GSM1112841
GEO; GSM1227087
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number24