Cellosaurus logo
expasy logo

Cellosaurus WAe009-A-R (CVCL_B5ZI)

[Text version]
Cell line name WAe009-A-R
Synonyms WA09 NRL+/eGFP; H9-NRL-GP
Accession CVCL_B5ZI
Resource Identification Initiative To cite this cell line use: WAe009-A-R (RRID:CVCL_B5ZI)
Comments Characteristics: Valuable tool for studying human rod development and disease and testing therapeutic strategies for retinitis pigmentosa (PubMed=29402929).
Characteristics: Using CRISPR/Cas9 a single allele of NRL was replaced with an eGFP reporter gene (PubMed=29402929).
Knockout cell: Method=CRISPR/Cas9; HGNC; 8002; NRL (Note=1 of 2 alleles).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=29402929; DOI=10.1038/s41598-018-20813-3
Phillips M.J., Capowski E.E., Petersen A., Jansen A.D., Barlow K., Edwards K.L., Gamm D.M.
Generation of a rod-specific NRL reporter line in human pluripotent stem cells.
Sci. Rep. 8:2370-2370(2018)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-R
Biological sample resources BioSamples; SAMEA113967375
Encyclopedic resources Wikidata; Q112041918
Entry history
Entry creation17-Mar-2022
Last entry update30-Jan-2024
Version number6