ID   WAe009-A-R
AC   CVCL_B5ZI
SY   WA09 NRL+/eGFP; H9-NRL-GP
DR   BioSamples; SAMEA113967375
DR   hPSCreg; WAe009-A-R
DR   Wikidata; Q112041918
RX   PubMed=29402929;
CC   Characteristics: Valuable tool for studying human rod development and disease and testing therapeutic strategies for retinitis pigmentosa (PubMed=29402929).
CC   Characteristics: Using CRISPR/Cas9 a single allele of NRL was replaced with an eGFP reporter gene (PubMed=29402929).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 8002; NRL (Note=1 of 2 alleles).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 17-03-22; Last updated: 30-01-24; Version: 6
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RX   PubMed=29402929; DOI=10.1038/s41598-018-20813-3;
RA   Phillips M.J., Capowski E.E., Petersen A., Jansen A.D., Barlow K.,
RA   Edwards K.L., Gamm D.M.;
RT   "Generation of a rod-specific NRL reporter line in human pluripotent
RT   stem cells.";
RL   Sci. Rep. 8:2370-2370(2018).
//