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Cellosaurus BTI-Tn-5B1-4 (CVCL_C190)

Cell line name BTI-Tn-5B1-4
Synonyms BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; High Five; High 5; High-5; High5; Hi-5; Hi5; Tn-5
Accession CVCL_C190
Resource Identification Initiative To cite this cell line use: BTI-Tn-5B1-4 (RRID:CVCL_C190)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: Infected with the alphanodavirus TnCLV.
Doubling time: 26 hours (PubMed=21667340).
Omics: Genome sequenced.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Hierarchy Parent: CVCL_Z410 (BTI-Tn-5B1-28)
CVCL_Z252 (BTI-Tnao38)CVCL_Z094 (H5CL-B)CVCL_Z095 (H5CL-F)
CVCL_RW20 (Hi-5 Rix4446)CVCL_UF19 (High5-ht33)CVCL_UF20 (High5-ht35)
CVCL_Z431 (Tn-4h)CVCL_RY32 (Tni-FNL)CVCL_RT23 (Tnms42)
Sex of cell Female
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/High_Five_cells

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

Granados R.R., Li G.-X., Derksen A.C.G., McKenna K.A.
A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus.
J. Invertebr. Pathol. 64:260-266(1994)

Granados R.R.
Trichoplusia ni cell line which supports replication of other baculoviruses.
Patent number US5300435, 05-Apr-1994

Yanase T., Yasunaga C., Kawarabata T.
Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
Acta Virol. 42:293-298(1998)

PubMed=10397817; DOI=10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
Saarinen M.A., Troutner K.A., Gladden S.G., Mitchell-Logean C.M., Murhammer D.W.
Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates.
Biotechnol. Bioeng. 63:612-617(1999)

Granados R.R., Li G.-X.
Clonal cell lines derived from BTI-TN-5B1-4.
Patent number US7179648, 20-Feb-2007

PubMed=17686877; DOI=10.1128/JVI.00807-07
Li T.-C., Scotti P.D., Miyamura T., Takeda N.
Latent infection of a new alphanodavirus in an insect cell line.
J. Virol. 81:10890-10896(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20300881; DOI=10.1007/s12033-010-9268-3
Krammer F., Schinko T., Palmberger D., Tauer C., Messner P., Grabherr R.
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)

PubMed=21667340; DOI=10.1007/s12250-011-3177-x
Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

Cell line collections ATCC; CRL-10859 - Discontinued
ECACC; 94020901 - Discontinued
Cell line databases/resources CLDB; cl4535
Lonza; 1180
TOKU-E; 4051
TOKU-E; 4129
Ontologies BTO; BTO:0003619
CLO; CLO_0009378
Other Wikidata; Q17126384