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Cellosaurus BTI-Tn-5B1-4 (CVCL_C190)

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Cell line name BTI-Tn-5B1-4
Synonyms BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5
Accession CVCL_C190
Resource Identification Initiative To cite this cell line use: BTI-Tn-5B1-4 (RRID:CVCL_C190)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: Infected with the alphanodavirus TnCLV.
Doubling time: 26 hours (PubMed=21667340); 34 +- 1 hours (in static culture), 27 +- 2 hours (in suspension culture) (PubMed=10585188).
Omics: Genome sequenced.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Hierarchy Parent: CVCL_Z410 (BTI-Tn-5B1-28)
CVCL_Z252 (BTI-Tnao38)CVCL_Z094 (H5CL-B)CVCL_Z095 (H5CL-F)
CVCL_RW20 (Hi-5 Rix4446)CVCL_UF19 (High5-ht33)CVCL_UF20 (High5-ht35)
CVCL_Z431 (Tn-4h)CVCL_RY32 (Tni-FNL)CVCL_RT23 (Tnms42)
Sex of cell Sex unspecified
Age at sampling Embryonic stage
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/High_Five_cells

PubMed=1369220; DOI=10.1021/bp00017a003
Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

Granados R.R., Li G.-X., Derksen A.C.G., McKenna K.A.
A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus.
J. Invertebr. Pathol. 64:260-266(1994)

Granados R.R.
Trichoplusia ni cell line which supports replication of other baculoviruses.
Patent number US5300435, 05-Apr-1994

Yanase T., Yasunaga C., Kawarabata T.
Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
Acta Virol. 42:293-298(1998)

PubMed=10397817; DOI=10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
Saarinen M.A., Troutner K.A., Gladden S.G., Mitchell-Logean C.M., Murhammer D.W.
Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates.
Biotechnol. Bioeng. 63:612-617(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Keith M.B., Farrell P.J., Iatrou K., Behie L.A.
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

Granados R.R., Li G.-X.
Clonal cell lines derived from BTI-TN-5B1-4.
Patent number US7179648, 20-Feb-2007

PubMed=17686877; DOI=10.1128/JVI.00807-07
Li T.-C., Scotti P.D., Miyamura T., Takeda N.
Latent infection of a new alphanodavirus in an insect cell line.
J. Virol. 81:10890-10896(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20300881; DOI=10.1007/s12033-010-9268-3
Krammer F., Schinko T., Palmberger D., Tauer C., Messner P., Grabherr R.
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)

PubMed=21667340; DOI=10.1007/s12250-011-3177-x
Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

PubMed=29376823; DOI=10.7554/eLife.31628
Fu Y., Yang Y., Zhang H., Farley G., Wang J., Quarles K.A., Weng Z., Zamore P.D.
The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology.
eLife 7:E31628-E31628(2018)

PubMed=30449074; DOI=10.1111/1755-0998.12966
Chen W.-B., Yang X.-W., Tetreau G., Song X.-Z., Coutu C., Hegedus D., Blissard G.W., Fei Z.-J., Wang P.
A high-quality chromosome-level genome assembly of a generalist herbivore, Trichoplusia ni.
Mol. Ecol. Resour. 19:485-496(2019)

Cell line collections ATCC; CRL-10859 - Discontinued
ECACC; 94020901 - Discontinued
Cell line databases/resources CLDB; cl4535
CCRID; 3142C0001000000319
Lonza; 1180
TOKU-E; 4051
TOKU-E; 4129
Ontologies BTO; BTO:0003619
CLO; CLO_0009378
Other Wikidata; Q17126384
Entry history
Entry creation06-Jun-2012
Last entry update02-Jul-2020
Version number22