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Cellosaurus AG-F (CVCL_D101)

Cell line name AG-F
Synonyms AGF
Accession CVCL_D101
Resource Identification Initiative To cite this cell line use: AG-F (RRID:CVCL_D101)
Comments Problematic cell line: Contaminated. Shown to be a CCRF-CEM derivative (PubMed=12592342; PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00171.
Doubling time: ~8-9 hours (Patent=US5480825).
Derived from sampling site: Peripheral blood.
Sequence variations Heterozygous for FLT3 p.Ala627Thr (c.1879G>A) (from parent cell line).
Heterozygous for KRAS p.Gly12Asp (c.35G>A) (from parent cell line).
Heterozygous for TP53 p.Arg175His (c.524G>A) and p.Arg248Gln (c.743G>A) (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0207 (CCRF-CEM)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO10,11,12
D13S31711,12
D16S53910,13,14
D5S81811,13,14,15
D7S8208,14.1
TH016,7
TPOX8,9
vWA16,17,18,19
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=AG-F
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

PubMed=8255104
Gazitt Y., He Y.-J., Shults K., Stelzer G.T., Cohen R.J.
Isolation and characterization of an early T-helper/inducer cell line with a unique pattern of surface phenotype, constitutive cytokine secretion and myc oncogene expression.
Leukemia 7:2034-2044(1993)

Patent=US5480825
Gazitt Y.
AG-F human T cell line with unique phenotype and cytokine secretions.
Patent number US5480825, 02-Jan-1996

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections ATCC; CRL-2530 - Discontinued
ATCC; CRL-11391
Biological sample resources BioSample; SAMN03151617
BioSample; SAMN03471608
Other Wikidata; Q54609413