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Cellosaurus 2fTGH-U5A (CVCL_D315)

Cell line name 2fTGH-U5A
Synonyms U5A; U-5A
Accession CVCL_D315
Resource Identification Initiative To cite this cell line use: 2fTGH-U5A (RRID:CVCL_D315)
Comments Population: Caucasian.
Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Transfected with: UniProtKB; P0A9M5; Escherichia coli gpt.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0115 (2fTGH)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC


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PubMed=7588638; DOI=10.1002/j.1460-2075.1995.tb00192.x
Lutfalla G., Holland S.J., Cinato E., Monneron D., Reboul J., Rogers N.C., Smith J.M., Stark G.R., Gardiner K., Mogensen K.E., Kerr I.M., Uze G.
Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.
EMBO J. 14:5100-5108(1995)

Cell line collections (Providers) CancerTools; 151808
ECACC; 12021506
Ximbio; 151808
Cell line databases/resources cancercelllines; CVCL_D315
Anatomy/cell type resources BTO; BTO:0006556
Encyclopedic resources Wikidata; Q54584482
Entry history
Entry creation22-Oct-2012
Last entry update05-Oct-2023
Version number21