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Cellosaurus 2fTGH-U5A (CVCL_D315)

[Text version]

Cell line name 2fTGH-U5A
Synonyms U5A
Accession CVCL_D315
Resource Identification Initiative To cite this cell line use: 2fTGH-U5A (RRID:CVCL_D315)
Comments Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Transfected with: UniProtKB; P0A9M5; E.coli gpt.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Sequence variations Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0115 (2fTGH)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S8209,10
D13S31712,14
D16S5399,12
TH016
TPOX8
vWA14,19

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Publications

PubMed=7588638; DOI=10.1002/j.1460-2075.1995.tb00192.x
Lutfalla G., Holland S.J., Cinato E., Monneron D., Reboul J., Rogers N.C., Smith J.M., Stark G.R., Gardiner K., Mogensen K.E., Kerr I.M., Uze G.
Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.
EMBO J. 14:5100-5108(1995)

Cross-references
Cell line collections ECACC; 12021506
Ximbio; 151808
Other Wikidata; Q54584482
Entry history
Entry creation22-Oct-2012
Last entry update20-May-2021
Version number15