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Cellosaurus HepG2/8F_HS (CVCL_D3XF)

[Text version]
Cell line name HepG2/8F_HS
Accession CVCL_D3XF
Resource Identification Initiative To cite this cell line use: HepG2/8F_HS (RRID:CVCL_D3XF)
Comments Population: Caucasian.
Characteristics: Could be useful as a new cell source for pharmacological studies and constructing bioartificial liver systems. When heat-stimulated it expresses 8 liver-specific transcription factor genes and thus many liver functions are induced upon heat treatment (PubMed=35406758).
Characteristics: Transduced with a pQMSCV-pHSP70B-TRE-pCMVmin-EGFP-IRES-tTA construct. The heat-activated HSPA6/HSP70B' promoter drives bicistronic gene expression of EGFP and of the tetracycline-controlled transactivator (tTA), which is mediated by an internal ribosomal entry site (IRES). Then, the tTA protein binds to TRE and activates the cytomegalovirus minimum promoter (pCMVmin) upstream of EGFP, which in turn induces further expression of the EGFP and tTA genes, providing a transcriptional positive feedback loop (from parent cell line).
Characteristics: The amplified tTA promotes the expression of 8 liver-enriched transcription factor (LETF) genes that were transduced using two constructs: PB513B-1/TRE-HNF1A-FOXA2/HNF3B-CEBPA-CEBPB-CEBPG-EF1a-PuroR and PB513B-1/TRE-HNF1B-HNF4A-ONECUT1-EF1a-Bla (PubMed=35406758).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: HGNC; 1833; CEBPA.
Transfected with: HGNC; 1834; CEBPB.
Transfected with: HGNC; 1837; CEBPG.
Transfected with: HGNC; 5022; FOXA2.
Transfected with: HGNC; 11621; HNF1A.
Transfected with: HGNC; 11630; HNF1B.
Transfected with: HGNC; 5024; HNF4A.
Transfected with: HGNC; 8138; ONECUT1.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D3XE (HepG2-HSP)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=35406758; DOI=10.3390/cells11071194
Kitano H., Kawabe Y., Kamihira M.
HepG2-based designer cells with heat-inducible enhanced liver functions.
Cells 11:1194.1-1194.16(2022)

Cross-references
Cell line collections (Providers) RCB; RCB5677
Entry history
Entry creation30-Jan-2024
Last entry update30-Jan-2024
Version number1