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Cellosaurus N27 (CVCL_D584)

[Text version]

Cell line name N27
Synonyms N-27; 1RB3AN27; 1RB3A-N27
Accession CVCL_D584
Resource Identification Initiative To cite this cell line use: N27 (RRID:CVCL_D584)
Comments Characteristics: Used as a dopaminergic neuron model for in vitro and in vivo studies (Millipore).
Characteristics: During many passages over 20 years, the original cell line became a mixture of cell types with less than 5% TH-positive dopamine neurons (PubMed=27512998).
Doubling time: 24 hours (PubMed=27512998).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV3-neo].
Derived from sampling site: Fetal brain; mesencephalon.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Hierarchy Children:
CVCL_ZJ56 (N27-A)
Sex of cell Sex unspecified
Age at sampling 12FD
Category Transformed cell line
Publications

PubMed=7820310; DOI=10.1007/BF02631258
Prasad K.N., Carvalho E., Kentroti S., Edwards-Prasad J., Freed C.R., Vernadakis A.
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
In Vitro Cell. Dev. Biol. Anim. 30:596-603(1994)

PubMed=27512998; DOI=10.1371/journal.pone.0160847
Gao L., Zhou W.-B., Symmes B., Freed C.R.
Re-cloning the N27 dopamine cell line to improve a cell culture model of Parkinson's disease.
PLoS ONE 11:E0160847-E0160847(2016)

Cross-references
Cell line collections Millipore; SCC048
Ontologies BTO; BTO:0002393
Other Wikidata; Q54907338
Entry history
Entry creation22-Oct-2012
Last entry update29-Oct-2020
Version number9