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Cellosaurus T-REx-293 (CVCL_D585)

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Cell line name T-REx-293
Synonyms T-REx 293; T-REx293; TRex-293; TREx293; T-REx-HEK293; T-REx HEK 293; HEK293-T-REx
Accession CVCL_D585
Resource Identification Initiative To cite this cell line use: T-REx-293 (RRID:CVCL_D585)
Comments Group: Vaccine production cell line.
Part of: ENCODE project common cell types; tier 3.
Biotechnology: Used for the production of the AstraZeneca SARS-CoV-2 (COVID-19) vaccine (Trade name: Vaxzevria; also known as ChAdOx1-SARS-COV-2 or AZD1222) (DrugBank; DB15656). In the context of the vaccine production, the tetR repressor blocks the expression of the SARS-CoV-2 spike protein gene during production of the recombinant adenoviral vector, thereby optimising vector production.
Transfected with: UniProtKB; P04483; E.coli Tn10 tetR.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from sampling site: Fetal kidney.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Children:
CVCL_U427 (Flp-In-T-REx-293)CVCL_YL74 (Jump-In T-REx HEK 293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages https://www.thermofisher.com/order/catalog/product/R71007
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/t/cell-lines-detail-410.html
http://genome.ucsc.edu/ENCODE/protocols/cell/human/T-REx-HEK293_Farnham_protocol.pdf
https://www.ema.europa.eu/en/documents/assessment-report/vaxzevria-previously-covid-19-vaccine-astrazeneca-epar-public-assessment-report_en.pdf
Cross-references
Cell line databases/resources CCRID; 3142C0001000000950
Ontologies BTO; BTO:0005238
CLO; CLO_0037238
Other Wikidata; Q54971367
Entry history
Entry creation22-Oct-2012
Last entry update20-May-2021
Version number16