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Cellosaurus Tau RD P301S FRET Biosensor (CVCL_DA04)

[Text version]
Cell line name Tau RD P301S FRET Biosensor
Accession CVCL_DA04
Resource Identification Initiative To cite this cell line use: Tau RD P301S FRET Biosensor (RRID:CVCL_DA04)
Comments Characteristics: Transduced with 2 separate lentivirus constructs encoding the tau repeat domain (RD) with the disease-associated p.Pro301Ser (p.Pro636Ser) mutation fused to either CFP or YFP (ATCC=CRL-3275).
Characteristics: Tau seeds introduced into the culture media of this cell line can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal which can be measured via microscopy, microplate readers or flow cytometry (ATCC=CRL-3275).
Transfected with: UniProtKB; Q66PV6; CFP.
Transfected with: UniProtKB; P42212; YFP (modified GFP at G-65, L-68, A-72, Y-203 and L-231).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11,12
D5S8188,9
D7S82011
D13S31712,14
D16S5399,13
TH017,9.3
TPOX11
vWA16,19

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Publications

PubMed=25261551; DOI=10.1073/pnas.1411649111
Holmes B.B., Furman J.L., Mahan T.E., Yamasaki T.R., Mirbaha H., Eades W.C., Belaygorod L., Cairns N.J., Holtzman D.M., Diamond M.I.
Proteopathic Tau seeding predicts tauopathy in vivo.
Proc. Natl. Acad. Sci. U.S.A. 111:E4376-E4385(2014)

PubMed=37553663; DOI=10.1186/s13024-023-00643-2
Lathuiliere A., Jo Y., Perbet R., Donahue C., Commins C., Quittot N., Fan Z.-Y., Bennett R.E., Hyman B.T.
Specific detection of Tau seeding activity in Alzheimer's disease using rationally designed biosensor cells.
Mol. Neurodegener. 18:53.1-53.13(2023)

Cross-references
Cell line collections (Providers) ATCC; CRL-3275
Encyclopedic resources Wikidata; Q54971737
Entry history
Entry creation13-Jul-2016
Last entry update05-Oct-2023
Version number9