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Cellosaurus H4L1.1c4 (CVCL_DC27)

Cell line name H4L1.1c4
Synonyms H4IIE.Luc
Accession CVCL_DC27
Resource Identification Initiative To cite this cell line use: H4L1.1c4 (RRID:CVCL_DC27)
Comments Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
Characteristics: Acts through the activation of a luciferase reporter gene under the control of dioxin-response elements (DREs).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Rat hepatocellular carcinoma (NCIt: C60416)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: AxC.
Hierarchy Parent: CVCL_0284 (H4-II-E)
Sex of cell Male
Category Cancer cell line
Publications

PubMed=8812260; DOI=10.1006/faat.1996.0152
Murk A.J., Legler J., Denison M.S., Giesy J.P., van de Guchte C., Brouwer A.
Chemical-activated luciferase gene expression (CALUX): a novel in vitro bioassay for Ah receptor active compounds in sediments and pore water.
Fundam. Appl. Toxicol. 33:149-160(1996)

PubMed=8812265; DOI=10.1006/faat.1996.0056
Garrison P.M., Tullis K., Aarts J.M.M.J.G., Brouwer A., Giesy J.P., Denison M.S.
Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals.
Fundam. Appl. Toxicol. 30:194-203(1996)

Patent=US5854010
Denison M.S., Brouwer A., Clark G.C.
Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor.
Patent number US5854010, 29-Dec-1998

PubMed=18969542; DOI=10.1016/j.talanta.2004.05.032
Denison M.S., Zhao B., Baston D.S., Clark G.C., Murata H., Han D.
Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals.
Talanta 63:1123-1133(2004)

PubMed=21394221; DOI=10.1007/s11426-010-0142-8
Zhao B., Baston D.S., Khan E., Sorrentino C., Denison M.S.
Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds.
Sci. China Chem. 53:1010-1016(2010)

PubMed=26366531; DOI=10.1021/acs.est.5b02906
Brennan J.C., He G.-C., Tsutsumi T., Zhao J., Wirth E., Fulton M.H., Denison M.S.
Development of species-specific Ah receptor-responsive third generation CALUX cell lines with enhanced responsiveness and improved detection limits.
Environ. Sci. Technol. 49:11903-11912(2015)

Cross-references
Encyclopedic resources Wikidata; Q54872158
Entry history
Entry creation13-Jul-2016
Last entry update29-Jun-2023
Version number7