ID   HG2L1.1c3
DR   Wikidata; Q54885924
RX   Patent=US5854010;
RX   PubMed=8812265;
CC   Population: Caucasian.
CC   Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
CC   Characteristics: Acts through the activation of a luciferase gene under the control of dioxin-response elements (DREs).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Derived from sampling site: Liver.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0027 ! Hep-G2
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 13-07-16; Last updated: 20-05-21; Version: 12
RX   Patent=US5854010;
RA   Denison M.S., Brouwer A., Clark G.C.;
RT   "Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and
RT   TCDD-like compounds and novel recombinant cell line useful therefor.";
RL   Patent number US5854010, 29-Dec-1998.
RX   PubMed=8812265; DOI=10.1006/faat.1996.0056;
RA   Garrison P.M., Tullis K., Aarts J.M.M.J.G., Brouwer A., Giesy J.P.,
RA   Denison M.S.;
RT   "Species-specific recombinant cell lines as bioassay systems for the
RT   detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals.";
RL   Fundam. Appl. Toxicol. 30:194-203(1996).