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Cellosaurus NMuMG/Fucci (CVCL_E069)

[Text version]
Cell line name NMuMG/Fucci
Synonyms NMuMG/Fucci #1; NMuMG-Fucci
Accession CVCL_E069
Resource Identification Initiative To cite this cell line use: NMuMG/Fucci (RRID:CVCL_E069)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange 2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric Azami-Green (mAG) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; Q60I24; Galaxea fascicularis monomeric Azami-Green.
Transfected with: UniProtKB; Q6I7B2; Fungia concinna monomeric Kusabira-Orange (with p.Lys49Glu, p.Pro70Val, p.Phe176Met, p.Lys185Glu, P.Lys188Glu, p.Ser192Asp, p.Ser196Gly and p.Leu210Gln = mKO2).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Namru.
Hierarchy Parent: CVCL_0075 (NMuMG)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=18267078; DOI=10.1016/j.cell.2007.12.033
Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H., Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T., Ogawa M., Masai H., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 132:487-498(2008)

Cross-references
Cell line collections (Providers) RCB; RCB2813
Cell line databases/resources CLO; CLO_0050967
Encyclopedic resources Wikidata; Q54930850
Entry history
Entry creation22-Oct-2012
Last entry update29-Jun-2023
Version number10