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Cellosaurus NMuMG/Fucci (CVCL_E069)

[Text version]

Cell line name NMuMG/Fucci
Synonyms NMuMG/Fucci #1; NMuMG-Fucci
Accession CVCL_E069
Resource Identification Initiative To cite this cell line use: NMuMG/Fucci (RRID:CVCL_E069)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange 2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric Azami-Green (mAG) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; Q60I24; Galaxea fascicularis monomeric Azami-Green.
Transfected with: UniProtKB; Q6I7B2; Fungia concinna monomeric Kusabira-Orange (with p.Lys49Glu, p.Pro70Val, p.Phe176Met, p.Lys185Glu, P.Lys188Glu, p.Ser192Asp, p.Ser196Gly and p.Leu210Gln = mKO2).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Namru.
Hierarchy Parent: CVCL_0075 (NMuMG)
Sex of cell Female
Category Spontaneously immortalized cell line

PubMed=18267078; DOI=10.1016/j.cell.2007.12.033
Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H., Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T., Ogawa M., Masai H., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 132:487-498(2008)

Cell line collections RCB; RCB2813
Ontologies CLO; CLO_0050967
Other Wikidata; Q54930850
Entry history
Entry creation22-Oct-2012
Last entry updated07-Sep-2018
Version number7