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Cellosaurus COS/Fucci (CVCL_E071)

[Text version]

Cell line name COS/Fucci
Synonyms COS-Fucci
Accession CVCL_E071
Resource Identification Initiative To cite this cell line use: COS/Fucci (RRID:CVCL_E071)
Comments Group: Non-human primate cell line.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange 2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric Azami-Green (mAG) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; Q60I24; Galaxea fascicularis monomeric Azami-Green.
Transfected with: UniProtKB; Q6I7B2; Fungia concinna monomeric Kusabira-Orange (with p.Lys49Glu, p.Pro70Val, p.Phe176Met, p.Lys185Glu, P.Lys188Glu, p.Ser192Asp, p.Ser196Gly and p.Leu210Gln = mKO2).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV6-1].
Species of origin Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) (NCBI Taxonomy: 9534)
Hierarchy Parent: CVCL_0224 (COS-7)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=18267078; DOI=10.1016/j.cell.2007.12.033
Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H., Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T., Ogawa M., Masai H., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 132:487-498(2008)

Cross-references
Cell line collections RCB; RCB2814
Ontologies CLO; CLO_0050516
Other Wikidata; Q54814317
Entry history
Entry creation22-Oct-2012
Last entry updated07-Sep-2018
Version number9