ID   COS/Fucci
AC   CVCL_E071
SY   COS-Fucci
DR   CLO; CLO_0050516
DR   RCB; RCB2814
DR   Wikidata; Q54814317
RX   PubMed=18267078;
CC   Group: Non-human primate cell line.
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
CC   Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange 2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric Azami-Green (mAG) to the ubiquitination domain of human geminin (aa 1-110).
CC   Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
CC   Transfected with: UniProtKB; Q60I24; Galaxea fascicularis monomeric Azami-Green.
CC   Transfected with: UniProtKB; Q6I7B2; Fungia concinna monomeric Kusabira-Orange (with p.Lys49Glu, p.Pro70Val, p.Phe176Met, p.Lys185Glu, P.Lys188Glu, p.Ser192Asp, p.Ser196Gly and p.Leu210Gln = mKO2).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV6-1].
CC   Derived from sampling site: Kidney.
OX   NCBI_TaxID=9534; ! Chlorocebus aethiops
HI   CVCL_0224 ! COS-7
SX   Male
AG   Adult
CA   Transformed cell line
DT   Created: 22-10-12; Last updated: 12-03-20; Version: 10
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RX   PubMed=18267078; DOI=10.1016/j.cell.2007.12.033;
RA   Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H.,
RA   Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T.,
RA   Ogawa M., Masai H., Miyawaki A.;
RT   "Visualizing spatiotemporal dynamics of multicellular cell-cycle
RT   progression.";
RL   Cell 132:487-498(2008).
//